Detailed information of TA system TA205286

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 39283..39537
• Replicon: plasmid pSA31IncF
• Accession: NZ_CP076277
• Organism: Escherichia coli strain SA31-1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: Q0H0B2
• Locus tag: A7A21_RS23900
• Protein ID: WP_001300273.1
• Coordinates: 39283..39432 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	39476..39537 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A21_RS23855 (35105)	35105..35362	-	258	WP_000523383.1	cloacin immunity family protein	-
A7A21_RS23860 (35372)	35372..35527	-	156	Protein_29	colicin E3/pyocin S6 family cytotoxin	-
A7A21_RS23865 (35765)	35765..36013	-	249	WP_257115041.1	hypothetical protein	-
A7A21_RS23870 (36108)	36108..36383	-	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	-
A7A21_RS23875 (36383)	36383..36667	-	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	-
A7A21_RS23880 (37580)	37580..38437	-	858	WP_000131011.1	incFII family plasmid replication initiator RepA	-
A7A21_RS23885 (38430)	38430..38504	-	75	WP_001365705.1	RepA leader peptide Tap	-
A7A21_RS23890 (38553)	38553..38678	+	126	WP_001436741.1	hypothetical protein	-
A7A21_RS23895 (38751)	38751..38999	-	249	WP_257115042.1	replication regulatory protein RepA	-
A7A21_RS23900 (39283)	39283..39432	-	150	WP_001300273.1	Hok/Gef family protein	Toxin
- (39476)	39476..39537	+	62	NuclAT_1	-	Antitoxin
- (39476)	39476..39537	+	62	NuclAT_1	-	Antitoxin
- (39476)	39476..39537	+	62	NuclAT_1	-	Antitoxin
- (39476)	39476..39537	+	62	NuclAT_1	-	Antitoxin
A7A21_RS23905 (39712)	39712..39846	-	135	WP_001058542.1	hypothetical protein	-
A7A21_RS23910 (40197)	40197..40658	-	462	WP_257115043.1	thermonuclease family protein	-
A7A21_RS23915 (41401)	41401..41604	-	204	WP_001327131.1	hypothetical protein	-
A7A21_RS23920 (41759)	41759..42316	-	558	WP_000139319.1	fertility inhibition protein FinO	-
A7A21_RS23925 (42419)	42419..43279	-	861	WP_257115044.1	alpha/beta hydrolase	-
A7A21_RS23930 (43338)	43338..44084	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..149410	149410
	-	flank	IS/Tn	-	-	33734..34933	1199

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5554.72 Da
• Isoelectric Point: 8.7678

>T205286 WP_001300273.1 NZ_CP076277:c39432-39283 [Escherichia coli]
MTKYALIGVLAVCATVLCFLLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T205286 NZ_CP101542:c2784100-2782778 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCTGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Nucleotide

• Length: 62 bp

>AT205286 NZ_CP076277:39476-39537 [Escherichia coli]
TTAAGGTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: Q0H0B2

Antitoxin