Detailed information of TA system TA205282

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4310807..4311409
• Replicon: chromosome
• Accession: NZ_CP076276
• Organism: Escherichia coli strain SA31-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: A7A21_RS21120
• Protein ID: WP_000897305.1
• Coordinates: 4310807..4311118 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A7A21_RS21125
• Protein ID: WP_000356397.1
• Coordinates: 4311119..4311409 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A21_RS21090 (4305837)	4305837..4306622	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
A7A21_RS21095 (4306721)	4306721..4307320	+	600	WP_001295269.1	glucose-1-phosphatase	-
A7A21_RS21100 (4307314)	4307314..4308186	+	873	WP_000920762.1	virulence factor BrkB family protein	-
A7A21_RS21105 (4308183)	4308183..4308620	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
A7A21_RS21110 (4308665)	4308665..4309606	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
A7A21_RS21115 (4309670)	4309670..4310578	-	909	WP_001162704.1	alpha/beta hydrolase	-
A7A21_RS21120 (4310807)	4310807..4311118	+	312	WP_000897305.1	hypothetical protein	Toxin
A7A21_RS21125 (4311119)	4311119..4311409	+	291	WP_000356397.1	NadS family protein	Antitoxin
A7A21_RS21130 (4312014)	4312014..4312232	+	219	WP_001295676.1	CopG family transcriptional regulator	-
A7A21_RS21135 (4312451)	4312451..4312693	+	243	WP_001086388.1	protein YiiF	-
A7A21_RS21140 (4313023)	4313023..4313952	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
A7A21_RS21145 (4313949)	4313949..4314584	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
A7A21_RS21150 (4314581)	4314581..4315483	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T205282 WP_000897305.1 NZ_CP076276:4310807-4311118 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T205282 NZ_CP101542:355949-356296 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT205282 WP_000356397.1 NZ_CP076276:4311119-4311409 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT205282 NZ_CP101542:356296-356595 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7