Detailed information of TA system TA205259

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 281230..281771
• Replicon: chromosome
• Accession: NZ_CP076276
• Organism: Escherichia coli strain SA31-1

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: E9TC49
• Locus tag: A7A21_RS01270
• Protein ID: WP_000615982.1
• Coordinates: 281230..281508 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: S1EWQ0
• Locus tag: A7A21_RS01275
• Protein ID: WP_000729704.1
• Coordinates: 281511..281771 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A21_RS01255 (278812)	278812..279390	+	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-
A7A21_RS01260 (279596)	279596..280363	+	768	WP_000333380.1	class II glutamine amidotransferase	-
A7A21_RS01265 (280334)	280334..281074	-	741	WP_001225679.1	peptidoglycan meso-diaminopimelic acid protein amidase	-
A7A21_RS01270 (281230)	281230..281508	-	279	WP_000615982.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
A7A21_RS01275 (281511)	281511..281771	-	261	WP_000729704.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
A7A21_RS01280 (281957)	281957..282730	+	774	WP_000543899.1	C40 family peptidase	-
A7A21_RS01285 (282787)	282787..283143	-	357	WP_001030484.1	type II toxin-antitoxin system HicB family antitoxin	-
A7A21_RS01290 (283136)	283136..283414	-	279	WP_000598760.1	type II toxin-antitoxin system HicA family toxin	-
A7A21_RS01295 (283519)	283519..285231	-	1713	Protein_251	flagellar biosynthesis protein FlhA	-
A7A21_RS01300 (285203)	285203..285988	+	786	WP_000207589.1	putative lateral flagellar export/assembly protein LafU	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-84)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10816.59 Da
• Isoelectric Point: 10.0702

>T205259 WP_000615982.1 NZ_CP076276:c281508-281230 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T205259 NZ_CP101533:743484-743969 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9391.77 Da
• Isoelectric Point: 5.0758

>AT205259 WP_000729704.1 NZ_CP076276:c281771-281511 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Nucleotide

• Length: 261 bp

>AT205259 NZ_CP101533:743194-743487 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0Y6Z6