Detailed information of TA system TA205254

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 87866..88287
• Replicon: plasmid pP393F10IncF
• Accession: NZ_CP076275
• Organism: Escherichia coli strain P393-F10

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: A7A10_RS25670
• Protein ID: WP_096937776.1
• Coordinates: 87866..87991 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	88089..88287 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A10_RS25630 (82901)	82901..83116	-	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-
A7A10_RS25635 (83252)	83252..83899	-	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
A7A10_RS25640 (84091)	84091..84474	-	384	WP_001063020.1	conjugal transfer relaxosome DNA-binding protein TraM	-
A7A10_RS25645 (84854)	84854..85444	+	591	WP_001375132.1	transglycosylase SLT domain-containing protein	-
A7A10_RS25650 (85740)	85740..86561	-	822	WP_257185976.1	DUF932 domain-containing protein	-
A7A10_RS25655 (86680)	86680..86967	-	288	WP_000107542.1	hypothetical protein	-
A7A10_RS25660 (86992)	86992..87198	-	207	WP_000547965.1	hypothetical protein	-
A7A10_RS25665 (87268)	87268..87565	+	298	Protein_90	hypothetical protein	-
A7A10_RS25670 (87866)	87866..87991	-	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
A7A10_RS25675 (87933)	87933..88082	-	150	Protein_92	DUF5431 family protein	-
- (88089)	88089..88287	-	199	NuclAT_0	-	Antitoxin
- (88089)	88089..88287	-	199	NuclAT_0	-	Antitoxin
- (88089)	88089..88287	-	199	NuclAT_0	-	Antitoxin
- (88089)	88089..88287	-	199	NuclAT_0	-	Antitoxin
A7A10_RS25680 (88256)	88256..89018	-	763	Protein_93	plasmid SOS inhibition protein A	-
A7A10_RS25685 (89015)	89015..89449	-	435	WP_257185946.1	conjugation system SOS inhibitor PsiB	-
A7A10_RS25690 (89504)	89504..91462	-	1959	WP_257185947.1	ParB/RepB/Spo0J family partition protein	-
A7A10_RS25695 (91528)	91528..91761	-	234	WP_000005990.1	DUF905 family protein	-
A7A10_RS25700 (91824)	91824..92363	-	540	WP_000290838.1	single-stranded DNA-binding protein	-
A7A10_RS25705 (92681)	92681..93190	+	510	WP_071532140.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..153041	153041

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T205254 WP_096937776.1 NZ_CP076275:c87991-87866 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T205254 NZ_CP101527:901664-902005 [Alkalimarinus sediminis]
ATGAAAGCAGTATTTGTAGAGTTGCCCCCGTTTGAGCGGGTTCGAAAAGATTATTGGGATGATAATACTTTTCGAAATTT
TCAGAATGAGATGCTTAATAATCCTTTTTCTGGAGATGTAATAAAAGGAACTGGAGGGCTTCGAAAGGTACGTTTTAGAG
ATGAGAAAAGACGCAAAGGAAAGCGAGGCGGTGTTAGAGTTATCTATTATTGGTGGAAGGAAGGAGCGCAGTTTTGGCTC
TTTACCATTTATGGTAAAGATGAAGTTACTGACCTAACAAACGCGGAAAGAAAGCTATTAGCTGAGTTGTTGAGGCATGA
AATACAAGTGAGGAGCTTATGA

Antitoxin

Nucleotide

• Length: 199 bp

>AT205254 NZ_CP076275:c88287-88089 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGCACAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin