Detailed information of TA system TA205244

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4451960..4452562
• Replicon: chromosome
• Accession: NZ_CP076274
• Organism: Escherichia coli strain P393-F10

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: A7A10_RS21970
• Protein ID: WP_000897305.1
• Coordinates: 4451960..4452271 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A7A10_RS21975
• Protein ID: WP_000356397.1
• Coordinates: 4452272..4452562 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A10_RS21940 (4446990)	4446990..4447775	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
A7A10_RS21945 (4447874)	4447874..4448473	+	600	WP_001295269.1	glucose-1-phosphatase	-
A7A10_RS21950 (4448467)	4448467..4449339	+	873	WP_000920762.1	virulence factor BrkB family protein	-
A7A10_RS21955 (4449336)	4449336..4449773	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
A7A10_RS21960 (4449818)	4449818..4450759	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
A7A10_RS21965 (4450823)	4450823..4451731	-	909	WP_001162704.1	alpha/beta hydrolase	-
A7A10_RS21970 (4451960)	4451960..4452271	+	312	WP_000897305.1	hypothetical protein	Toxin
A7A10_RS21975 (4452272)	4452272..4452562	+	291	WP_000356397.1	NadS family protein	Antitoxin
A7A10_RS21980 (4453167)	4453167..4453385	+	219	WP_001315930.1	CopG family transcriptional regulator	-
A7A10_RS21985 (4453605)	4453605..4453847	+	243	WP_001087409.1	protein YiiF	-
A7A10_RS21990 (4454177)	4454177..4455106	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
A7A10_RS21995 (4455103)	4455103..4455738	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
A7A10_RS22000 (4455735)	4455735..4456637	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T205244 WP_000897305.1 NZ_CP076274:4451960-4452271 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T205244 NZ_CP101519:1052007-1052261 [Ensifer adhaerens]
ATGAACGGCAAGCAGAAAAAGACTCTCGTGGCGGTTTTCAAAGATCCGGTCTCGGGAACCATAGAGTGGGCCGGTATAGA
AAACCTGCTTGTCGCTGCCGGCGCGAACGTCATCGAAAGAAACGGCTCGCGCGTTAAGTTCGAAAAGGACGGCATAATCG
CGAGCTTCCATCGTCCTCATCCCGACAAGGAGGCAAAACGTTATCAAGTTTGCGATGCTCGCGAATTCCTGACGCAAATT
GGAGTAGAACCATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT205244 WP_000356397.1 NZ_CP076274:4452272-4452562 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT205244 NZ_CP101519:1052258-1052614 [Ensifer adhaerens]
ATGAACACCATGACTTACAACGGTTATCACGCTCGCATAGAATTCGACGCGGAGGACGAGCTGTTCGTCGGTAAGATTGC
TGGCATTTCGGACGTAGTCGGGTTTCACGGGGATAGCGTTTCTGAACTGAAGAAGGCGTTCCATGAGGCTGTCGATGATT
ACCTTGAGACCTGCAAGAGCATCGGCAAAGAGCCTCAAAGACCCTATTCCGGCAAGATGATGTTTCGAGTTGCTCCCGAA
GTGCATCGCCGAGCTTCTCTGGCTGCCGAACTGTCGGGGAAGAGCCTCAATCAGTGGGCCGAGGAGGCATTAGACGAAGC
CGCGGCGCACGTCGCAGAGGCCCGCCTTTCGGCATAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7