205211

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4677762..4678259 Replicon chromosome
Accession NZ_CP076272
Organism Escherichia coli strain E21845/O/A

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag A7A11_RS22875 Protein ID WP_001173343.1
Coordinates 4678086..4678259 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag A7A11_RS22870 Protein ID WP_000398619.1
Coordinates 4677762..4678058 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A11_RS22850 (4674493) 4674493..4675212 - 720 WP_000611288.1 two-component system response regulator DcuR -
A7A11_RS22855 (4675209) 4675209..4676840 - 1632 WP_113441013.1 sensor histidine kinase -
A7A11_RS22860 (4677021) 4677021..4677251 + 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
A7A11_RS22865 (4677263) 4677263..4677535 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
A7A11_RS22870 (4677762) 4677762..4678058 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
A7A11_RS22875 (4678086) 4678086..4678259 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
A7A11_RS22880 (4678378) 4678378..4679895 - 1518 WP_257191800.1 lysine--tRNA ligase -
A7A11_RS22885 (4680132) 4680132..4681589 - 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4677762..4687113 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T205211 WP_001173343.1 NZ_CP076272:4678086-4678259 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T205211 NZ_CP101497:1664495-1664881 [Microcella sp. MMS21-STM10]
GTGCTGTATCTCGATACGTCTGCCGCGGTGAAGCTTCTGATCGAGGAAAAGGAGTCTTCGGCACTTCGGGCGTACCTGAG
CCACCACGACTGGGCTTCGAGCGCACTCGTGCGAACCGAGCTGGTGCGGGCACTCCTTCGCGCCGATCCCTCCGTTGTGC
CACGGGCGCTCGACCTGCTCGCGCAGGGCAACCTGCTGGTCATCGACACCCAGGTGCTCGACACGGCAGCGCGACTGTCG
CCGCCATCACTGCGCAGCCTCGACGCGATTCACCTCGCGAGCGCGCTGGAGCTTCGCGACGAGTTGACCGCGTTCGTTGC
GTACGACGAGCGACTGCTGGCCGCGGCATCCGCGCTGGGTATGCCGGTCGCGTCGCCGGCCGATTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT205211 WP_000398619.1 NZ_CP076272:4677762-4678058 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT205211 NZ_CP101497:1664198-1664494 [Microcella sp. MMS21-STM10]
ATGGACCGGATCGGGGTGCGCGAGCTGCGGCAGAACGCGAGCAAGTACCTTGACCGCGTGAAGCAAGGCGAGTCGATCGA
GGTGACCGAGCGTGGGGTGCCGGTCGCGATCATCGGGCCCGTGCCGACAGTGAAAGATTCGCGAGTGCAAGAACTGATTG
ACGAAAGGCGCATGACGCCGGGGCGCGGGGGCTCTGCCGCGTGGCTCGCCGCGAATCCTCCCACCCCGATCAACCCGGAG
CACGGCAGGCCGTCGATCGCTGATCTTCTCGATGAACAACGCGAAGAGCGGCTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References