Detailed information of TA system TA205210

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4399868..4400470
• Replicon: chromosome
• Accession: NZ_CP076272
• Organism: Escherichia coli strain E21845/O/A

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: A7A11_RS21650
• Protein ID: WP_000897305.1
• Coordinates: 4399868..4400179 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A7A11_RS21655
• Protein ID: WP_000356397.1
• Coordinates: 4400180..4400470 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A11_RS21620 (4394898)	4394898..4395683	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
A7A11_RS21625 (4395782)	4395782..4396381	+	600	WP_001295269.1	glucose-1-phosphatase	-
A7A11_RS21630 (4396375)	4396375..4397247	+	873	WP_000920762.1	virulence factor BrkB family protein	-
A7A11_RS21635 (4397244)	4397244..4397681	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
A7A11_RS21640 (4397726)	4397726..4398667	+	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
A7A11_RS21645 (4398731)	4398731..4399639	-	909	WP_001162704.1	alpha/beta hydrolase	-
A7A11_RS21650 (4399868)	4399868..4400179	+	312	WP_000897305.1	hypothetical protein	Toxin
A7A11_RS21655 (4400180)	4400180..4400470	+	291	WP_000356397.1	NadS family protein	Antitoxin
A7A11_RS21660 (4401075)	4401075..4401293	+	219	WP_001295676.1	CopG family transcriptional regulator	-
A7A11_RS21665 (4401512)	4401512..4401754	+	243	WP_001086388.1	protein YiiF	-
A7A11_RS21670 (4401983)	4401983..4402963	-	981	WP_000399648.1	IS110-like element IS621 family transposase	-
A7A11_RS21675 (4403362)	4403362..4404291	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
A7A11_RS21680 (4404288)	4404288..4404923	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4401983..4402963	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T205210 WP_000897305.1 NZ_CP076272:4399868-4400179 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T205210 NZ_CP101497:c388530-388165 [Microcella sp. MMS21-STM10]
ATGCTGGTTGACACCTCGGTCTGGATCGACCATCTGCACCGCGGGGAGAGGGAGCTCGTGCGTGCATTGAACACATCGCT
GGTCGTCCAGCACCCGATGGTCATCGGAGAGTTGTCCTTGGGGAGACTCCGCGACCGGTCGGTGGTGCTTGAGTTGCTGG
GCAACCTTCCGAGCTCACCGATCGCGAGTCACAGCGAAGTCGCCGGTTTCGTCGAGGTTCATGCGCTCTACGGTCAGGGG
CTGAGTCTTGTTGACGCGCACCTGCTCGCATCCGTCGTGCTCGCGCCGGGCACGACGCTGTGGACTCGGGACAAGCGCCT
GCGCGAAGCGGCCAACCGGCTCGGGGTCGGCGCGAGCCTCCCGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT205210 WP_000356397.1 NZ_CP076272:4400180-4400470 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT205210 NZ_CP101497:c388731-388537 [Microcella sp. MMS21-STM10]
ATGCGCACGACGGTGAATCTCGACGATGACCTGCTGGCCAAAGCCGCAGAGCTCAGCGGCACGACCGAACGCACGGCCCT
GCTGCGCGAGAGTCTGCAGGCCCTCATTGAGCGGGAGAGCGCTCGGCGGCTCAGTCTTCTCGGCGGCACCGACGCCGGTG
CGACCGCCGCAGATCGTCACCGCGACGTCGCCTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7