Detailed information of TA system TA205207

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3608632..3609431
• Replicon: chromosome
• Accession: NZ_CP076272
• Organism: Escherichia coli strain E21845/O/A

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: U9XVR9
• Locus tag: A7A11_RS17800
• Protein ID: WP_000347267.1
• Coordinates: 3608967..3609431 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: A7A11_RS17795
• Protein ID: WP_001307405.1
• Coordinates: 3608632..3608967 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A11_RS17780 (3604417)	3604417..3605187	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
A7A11_RS17785 (3605203)	3605203..3606537	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
A7A11_RS17790 (3606912)	3606912..3608483	+	1572	WP_001273741.1	galactarate dehydratase	-
A7A11_RS17795 (3608632)	3608632..3608967	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
A7A11_RS17800 (3608967)	3608967..3609431	+	465	WP_000347267.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
A7A11_RS17805 (3609486)	3609486..3610295	-	810	WP_000072180.1	aga operon transcriptional regulator AgaR	-
A7A11_RS17810 (3610544)	3610544..3611824	+	1281	WP_000681947.1	tagatose-bisphosphate aldolase subunit KbaZ	-
A7A11_RS17815 (3611847)	3611847..3612320	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
A7A11_RS17820 (3612331)	3612331..3613110	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
A7A11_RS17825 (3613100)	3613100..3613978	+	879	WP_001298758.1	PTS N-acetylgalactosamine transporter subunit IID	-
A7A11_RS17830 (3613996)	3613996..3614430	+	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3597870..3609431	11561

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17807.17 Da
• Isoelectric Point: 9.4947

>T205207 WP_000347267.1 NZ_CP076272:3608967-3609431 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T205207 NZ_CP101497:c42274-41963 [Microcella sp. MMS21-STM10]
ATGTACGAGCTGCGCAGAAGTGCTGAGTTCGACCGGTGGTTGGCCAGGCTGAGCGACCGTGGTGCTGTTGCGCGGGTCTT
GGTACGGCTTGATCGCCTTGCACGCGGCAACCCCGGCGACGTTCGACCCGTTGGCTTCGGTGTATCGGAGCTACGGATCG
ACCATGGACCCGGTCTTCGCGTGTACTACCTGCAACACGGCGGCGCCATAGTGCTGCTGCTGTGCGGGGGTGACAAGTCG
ACTCAGTCGCGAGACATCAGGCGGGCACATCGTGTGGCCGAGCAATGGAGGGGAGAGAAGCATGACGACTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT205207 WP_001307405.1 NZ_CP076272:3608632-3608967 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT205207 NZ_CP101497:c41973-41677 [Microcella sp. MMS21-STM10]
ATGACGACTGAGAGGTTCAGCCAATTCGATGCTGCCGACTATCTGTCCGACATGGAGACCGCGGCGGCCTATCTCGAAGC
GACGATCGCCGAGAGCGGCGACGACCCTGCCACCATTTCGGCTGCGCTCGGGGCCATCGCCCGCTCGGGCAACCTCAGCG
AGCTGGCTCGGCGCACCGGGATGAGCCGCGAGGGGCTTTACAAGGCGCTCTCCGCCGACGGGAATCCGAGCTTCGCGACC
ATTGTCAAGGTGTCGAGGGCGCTCGGGCTCCGGTTGGAGTTTCACGCCATCGCCTAG

Structures

Toxin

• AlphaFold DB: A0A0E0XTR4

Antitoxin

• AlphaFold DB: A0A0E0XVC7