Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 282994..283535 Replicon chromosome
Accession NZ_CP076272
Organism Escherichia coli strain E21845/O/A

Toxin (Protein)


Gene name yafQ Uniprot ID A7ZHY5
Locus tag A7A11_RS01265 Protein ID WP_012000916.1
Coordinates 282994..283272 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag A7A11_RS01270 Protein ID WP_000729704.1
Coordinates 283275..283535 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A11_RS01250 (280576) 280576..281154 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
A7A11_RS01255 (281360) 281360..282127 + 768 WP_000333380.1 class II glutamine amidotransferase -
A7A11_RS01260 (282098) 282098..282838 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
A7A11_RS01265 (282994) 282994..283272 - 279 WP_012000916.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
A7A11_RS01270 (283275) 283275..283535 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
A7A11_RS01275 (283721) 283721..284494 + 774 WP_000543899.1 C40 family peptidase -
A7A11_RS01280 (284551) 284551..284907 - 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
A7A11_RS01285 (284900) 284900..285178 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
A7A11_RS01290 (285283) 285283..287376 - 2094 WP_089620480.1 flagellar biosynthesis protein FlhA -
A7A11_RS01295 (287360) 287360..288499 - 1140 WP_000785662.1 flagellar type III secretion system protein FlhB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T205186 WP_012000916.1 NZ_CP076272:c283272-282994 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQSSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T205186 NZ_CP101416:c2208591-2208256 [Pseudanabaena sp. Chao 1811]
ATGGTAAAGCAATACATTCCAGATCGTGGTGATATTGTTTGGTTAGATTTCGATCCACAGGCTGGGCATGAACAATCTGG
GCATCGTCCTGCTTTTGTGATTTCGCCGATCGCCTATAATCAAAGGTCAAATTTGGCTCTGCTTTGTCCGATTACTTCTA
AAGTTAAGGGATGGAAGTTTGAGGTTCTACTCACGGATACTAAGACTAAGGGAGTGATTCTTGCGGATCAGATTAAGAGT
TTGGATTGGCAAGCGAGAAGGATCGATTTTATTGAGAAGGCTTCTGTAAGTATTATTGATGAAGTTATCGGCAAAATTGA
GGCGTTATTAACCTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT205186 WP_000729704.1 NZ_CP076272:c283535-283275 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT205186 NZ_CP101416:c2208827-2208585 [Pseudanabaena sp. Chao 1811]
ATGACTGCCGCGATCGCGAAGTGGGGTAATAGTTTAGCTGTACGCATTCCGCAAGCTATAGCTGAACAGGTACAAATTCA
GGCTGGAAGCGAAATCAATATTGAGATTATTGATGGCAAGATTGTTTTGACACCGCATCGACGTAAAAAATACACTCTAG
ATGAATTGCTGGATGGGATGACTTCCGAGCATCTCCATGCAGAAACTTCTACGGGTGTTTCTGTTGGGAATGAGGCATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A7ZHY5

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References