Detailed information of TA system TA205181

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 77650..78071
• Replicon: plasmid pCeylon31IncF
• Accession: NZ_CP076271
• Organism: Escherichia coli strain Ceylon31

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: A7A14_RS24140
• Protein ID: WP_096937776.1
• Coordinates: 77650..77775 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	77873..78071 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A14_RS24100 (72726)	72726..72953	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
A7A14_RS24105 (73047)	73047..73733	-	687	WP_000332492.1	PAS domain-containing protein	-
A7A14_RS24110 (73923)	73923..74306	-	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
A7A14_RS24115 (74583)	74583..75230	+	648	WP_122990291.1	transglycosylase SLT domain-containing protein	-
A7A14_RS24120 (75526)	75526..76347	-	822	WP_032257923.1	DUF932 domain-containing protein	-
A7A14_RS24125 (76466)	76466..76752	-	287	Protein_79	hypothetical protein	-
A7A14_RS24130 (76777)	76777..76983	-	207	WP_034174079.1	hypothetical protein	-
A7A14_RS24135 (77053)	77053..77349	+	297	Protein_81	hypothetical protein	-
A7A14_RS24140 (77650)	77650..77775	-	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
A7A14_RS24145 (77717)	77717..77866	-	150	Protein_83	DUF5431 family protein	-
- (77873)	77873..78071	-	199	NuclAT_0	-	Antitoxin
- (77873)	77873..78071	-	199	NuclAT_0	-	Antitoxin
- (77873)	77873..78071	-	199	NuclAT_0	-	Antitoxin
- (77873)	77873..78071	-	199	NuclAT_0	-	Antitoxin
A7A14_RS24150 (78040)	78040..78802	-	763	Protein_84	plasmid SOS inhibition protein A	-
A7A14_RS24155 (78799)	78799..79233	-	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
A7A14_RS24160 (79288)	79288..81246	-	1959	WP_160521604.1	ParB/RepB/Spo0J family partition protein	-
A7A14_RS24165 (81312)	81312..81545	-	234	WP_000005990.1	DUF905 family protein	-
A7A14_RS24170 (81602)	81602..82141	-	540	WP_000290801.1	single-stranded DNA-binding protein	-
A7A14_RS24175 (82459)	82459..82968	+	510	WP_071600123.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	pefD / pefC / pefA	1..127140	127140

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T205181 WP_096937776.1 NZ_CP076271:c77775-77650 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T205181 NZ_CP101416:136473-136724 [Pseudanabaena sp. Chao 1811]
ATGAATGCAGTTACTTATACCTATGTCCGCAATAATCTTGCGAAAACCTTAGAGAAGGTTTGTGATGATCACGATCCTGT
AATTGTGACCCGTCAAAATCAAAATTCAGTAGTGATTATGTCACTTGAGGACTACGAAGCTCTTGCAGAAACTGCCTATT
TGTTGCGTAGCCCCCAAAATGCTCAGAGGTTAATTAGGGCGATCGCTGATCTAGAATCTGGTAAGGGCAAGCAGAGAGAA
CTTGTCGAATGA

Antitoxin

Nucleotide

• Length: 199 bp

>AT205181 NZ_CP076271:c78071-77873 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin