Detailed information of TA system TA205176

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4333538..4334140
• Replicon: chromosome
• Accession: NZ_CP076270
• Organism: Escherichia coli strain Ceylon31

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: A7A14_RS21085
• Protein ID: WP_000897305.1
• Coordinates: 4333538..4333849 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A7A14_RS21090
• Protein ID: WP_000356397.1
• Coordinates: 4333850..4334140 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A14_RS21060 (4329452)	4329452..4330051	+	600	WP_257122423.1	glucose-1-phosphatase	-
A7A14_RS21065 (4330045)	4330045..4330917	+	873	WP_000920762.1	virulence factor BrkB family protein	-
A7A14_RS21070 (4330914)	4330914..4331351	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
A7A14_RS21075 (4331396)	4331396..4332337	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
A7A14_RS21080 (4332401)	4332401..4333309	-	909	WP_001162704.1	alpha/beta hydrolase	-
A7A14_RS21085 (4333538)	4333538..4333849	+	312	WP_000897305.1	hypothetical protein	Toxin
A7A14_RS21090 (4333850)	4333850..4334140	+	291	WP_000356397.1	NadS family protein	Antitoxin
A7A14_RS21095 (4334745)	4334745..4334963	+	219	WP_001295676.1	CopG family transcriptional regulator	-
A7A14_RS21100 (4335182)	4335182..4335424	+	243	WP_001087409.1	protein YiiF	-
A7A14_RS21105 (4335754)	4335754..4336683	-	930	WP_000027706.1	formate dehydrogenase accessory protein FdhE	-
A7A14_RS21110 (4336680)	4336680..4337315	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
A7A14_RS21115 (4337312)	4337312..4338214	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T205176 WP_000897305.1 NZ_CP076270:4333538-4333849 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T205176 NZ_CP101390:4783099-4783410 [Salmonella enterica]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATACTATCGGGGTAATAAAGAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT205176 WP_000356397.1 NZ_CP076270:4333850-4334140 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT205176 NZ_CP101390:4783407-4783853 [Salmonella enterica]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTCAAACTACCAGCAGATGCCTTCATCGAGTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7