Detailed information of TA system TA205149

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 14215..14636
• Replicon: plasmid pD217IncF
• Accession: NZ_CP076269
• Organism: Escherichia coli strain D217-5

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: A7A16_RS23205
• Protein ID: WP_096937776.1
• Coordinates: 14511..14636 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	14215..14413 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A16_RS23170 (9318)	9318..9827	-	510	WP_071532140.1	hypothetical protein	-
A7A16_RS23175 (10145)	10145..10618	+	474	WP_257109156.1	single-stranded DNA-binding protein	-
A7A16_RS23180 (10741)	10741..10974	+	234	WP_000005990.1	DUF905 family protein	-
A7A16_RS23185 (11040)	11040..12998	+	1959	WP_000117171.1	ParB/RepB/Spo0J family partition protein	-
A7A16_RS23190 (13053)	13053..13487	+	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
A7A16_RS23195 (13484)	13484..14246	+	763	Protein_17	plasmid SOS inhibition protein A	-
- (14215)	14215..14413	+	199	NuclAT_0	-	Antitoxin
- (14215)	14215..14413	+	199	NuclAT_0	-	Antitoxin
- (14215)	14215..14413	+	199	NuclAT_0	-	Antitoxin
- (14215)	14215..14413	+	199	NuclAT_0	-	Antitoxin
A7A16_RS23200 (14420)	14420..14569	+	150	Protein_18	DUF5431 family protein	-
A7A16_RS23205 (14511)	14511..14636	+	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
A7A16_RS23210 (14937)	14937..15234	-	298	Protein_20	hypothetical protein	-
A7A16_RS23215 (15304)	15304..15510	+	207	WP_000547965.1	hypothetical protein	-
A7A16_RS23220 (15535)	15535..15822	+	288	WP_000107542.1	hypothetical protein	-
A7A16_RS23225 (15941)	15941..16762	+	822	WP_001234475.1	DUF932 domain-containing protein	-
A7A16_RS23230 (17058)	17058..17648	-	591	WP_001375132.1	transglycosylase SLT domain-containing protein	-
A7A16_RS23235 (17999)	17999..18382	+	384	WP_001063021.1	conjugal transfer relaxosome DNA-binding protein TraM	-
A7A16_RS23240 (18573)	18573..19220	+	648	WP_000332519.1	conjugal transfer transcriptional regulator TraJ	-
A7A16_RS23245 (19356)	19356..19571	+	216	WP_000086381.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..88268	88268

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T205149 WP_096937776.1 NZ_CP076269:14511-14636 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T205149 NZ_CP101388:871261-871674 [Salmonella enterica]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTAA

Antitoxin

Nucleotide

• Length: 199 bp

>AT205149 NZ_CP076269:14215-14413 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin