Detailed information of TA system TA205146

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4244445..4245047
• Replicon: chromosome
• Accession: NZ_CP076268
• Organism: Escherichia coli strain D217-5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: A7A16_RS20625
• Protein ID: WP_000897305.1
• Coordinates: 4244445..4244756 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A7A16_RS20630
• Protein ID: WP_000356397.1
• Coordinates: 4244757..4245047 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A16_RS20595 (4239475)	4239475..4240260	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
A7A16_RS20600 (4240359)	4240359..4240958	+	600	WP_001295269.1	glucose-1-phosphatase	-
A7A16_RS20605 (4240952)	4240952..4241824	+	873	WP_000920762.1	virulence factor BrkB family protein	-
A7A16_RS20610 (4241821)	4241821..4242258	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
A7A16_RS20615 (4242303)	4242303..4243244	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
A7A16_RS20620 (4243308)	4243308..4244216	-	909	WP_001162704.1	alpha/beta hydrolase	-
A7A16_RS20625 (4244445)	4244445..4244756	+	312	WP_000897305.1	hypothetical protein	Toxin
A7A16_RS20630 (4244757)	4244757..4245047	+	291	WP_000356397.1	NadS family protein	Antitoxin
A7A16_RS20635 (4245633)	4245633..4245851	+	219	WP_001315930.1	CopG family transcriptional regulator	-
A7A16_RS20640 (4246071)	4246071..4246313	+	243	WP_001087409.1	protein YiiF	-
A7A16_RS20645 (4246643)	4246643..4247572	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
A7A16_RS20650 (4247569)	4247569..4248204	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
A7A16_RS20655 (4248201)	4248201..4249103	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T205146 WP_000897305.1 NZ_CP076268:4244445-4244756 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T205146 NZ_CP101388:206805-207290 [Salmonella enterica]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT205146 WP_000356397.1 NZ_CP076268:4244757-4245047 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT205146 NZ_CP101388:206530-206817 [Salmonella enterica]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7