Detailed information of TA system TA205140

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2266612..2267114
• Replicon: chromosome
• Accession: NZ_CP076268
• Organism: Escherichia coli strain D217-5

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: A7A16_RS11225
• Protein ID: WP_000767829.1
• Coordinates: 2266612..2266866 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: A7A16_RS11230
• Protein ID: WP_001259255.1
• Coordinates: 2266863..2267114 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A16_RS11195 (2261627)	2261627..2261854	-	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-
A7A16_RS11200 (2261868)	2261868..2262926	-	1059	WP_000492346.1	thiosulfate utilization transporter TsuA/YeeE	-
A7A16_RS11205 (2263105)	2263105..2264463	-	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
A7A16_RS11210 (2264453)	2264453..2264515	-	63	WP_010723108.1	membrane protein YoeI	-
A7A16_RS11215 (2264730)	2264730..2265659	-	930	WP_000803351.1	LysR substrate-binding domain-containing protein	-
A7A16_RS11220 (2265705)	2265705..2266529	-	825	WP_021562277.1	SDR family oxidoreductase	-
A7A16_RS11225 (2266612)	2266612..2266866	-	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
A7A16_RS11230 (2266863)	2266863..2267114	-	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
A7A16_RS11235 (2267397)	2267397..2267447	+	51	WP_001364200.1	his operon leader peptide	-
A7A16_RS11240 (2267593)	2267593..2268492	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
A7A16_RS11245 (2268498)	2268498..2269802	+	1305	WP_001570037.1	histidinol dehydrogenase	-
A7A16_RS11250 (2269799)	2269799..2270869	+	1071	WP_000108962.1	histidinol-phosphate transaminase	-
A7A16_RS11255 (2270869)	2270869..2271936	+	1068	WP_000080095.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T205140 WP_000767829.1 NZ_CP076268:c2266866-2266612 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T205140 NZ_CP101386:c4191448-4191161 [Salmonella enterica]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGTTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCCCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT205140 WP_001259255.1 NZ_CP076268:c2267114-2266863 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT205140 NZ_CP101386:c4191174-4190872 [Salmonella enterica]
ATGAGCATGGTTAAACATAAACGCGGCAACGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• AlphaFold DB: A0A7U9LNR0