Detailed information of TA system TA205114

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 52411..52665
• Replicon: plasmid pNADC1036IncF
• Accession: NZ_CP076265
• Organism: Escherichia coli strain NADC1036

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: Q0H0B2
• Locus tag: A7A17_RS24155
• Protein ID: WP_001300273.1
• Coordinates: 52411..52560 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	52604..52665 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A17_RS24115 (47770)	47770..48261	-	492	WP_225403467.1	hypothetical protein	-
A7A17_RS24120 (49163)	49163..49450	-	288	WP_000865085.1	helix-turn-helix transcriptional regulator	-
A7A17_RS24125 (49450)	49450..49761	-	312	WP_000483535.1	type II toxin-antitoxin system RelE/ParE family toxin	-
A7A17_RS24130 (49867)	49867..50007	-	141	WP_001315588.1	hypothetical protein	-
A7A17_RS24135 (50708)	50708..51565	-	858	WP_000131011.1	incFII family plasmid replication initiator RepA	-
A7A17_RS24140 (51558)	51558..51632	-	75	WP_001365705.1	RepA leader peptide Tap	-
A7A17_RS24145 (51681)	51681..51806	+	126	WP_094259391.1	replication protein RepA	-
A7A17_RS24150 (51879)	51879..52127	-	249	WP_000083838.1	replication regulatory protein RepA	-
A7A17_RS24155 (52411)	52411..52560	-	150	WP_001300273.1	Hok/Gef family protein	Toxin
- (52604)	52604..52665	+	62	NuclAT_1	-	Antitoxin
- (52604)	52604..52665	+	62	NuclAT_1	-	Antitoxin
- (52604)	52604..52665	+	62	NuclAT_1	-	Antitoxin
- (52604)	52604..52665	+	62	NuclAT_1	-	Antitoxin
A7A17_RS24160 (52840)	52840..52974	-	135	WP_001058542.1	hypothetical protein	-
A7A17_RS24165 (53325)	53325..53786	-	462	WP_000760079.1	thermonuclease family protein	-
A7A17_RS24170 (54530)	54530..54733	-	204	WP_040073114.1	hypothetical protein	-
A7A17_RS24175 (54888)	54888..55445	-	558	WP_047649932.1	fertility inhibition protein FinO	-
A7A17_RS24180 (55548)	55548..56408	-	861	WP_000704529.1	alpha/beta hydrolase	-
A7A17_RS24185 (56467)	56467..57207	-	741	WP_094259392.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	cdtC / cdtB / cdtA / cnf1 / hlyC / iutA / iucD / iucC / iucB / iucA / f17d-G / f17d-C / f17d-D / f17d-A	1..145019	145019

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5554.72 Da
• Isoelectric Point: 8.7678

>T205114 WP_001300273.1 NZ_CP076265:c52560-52411 [Escherichia coli]
MTKYALIGVLAVCATVLCFLLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T205114 NZ_CP101382:206805-207290 [Salmonella enterica]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Nucleotide

• Length: 62 bp

>AT205114 NZ_CP076265:52604-52665 [Escherichia coli]
TTAAGGTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: Q0H0B2

Antitoxin