Detailed information of TA system TA205111

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4343049..4343651
• Replicon: chromosome
• Accession: NZ_CP076264
• Organism: Escherichia coli strain NADC1036

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: A7A17_RS21335
• Protein ID: WP_000897305.1
• Coordinates: 4343049..4343360 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A7A17_RS21340
• Protein ID: WP_000356397.1
• Coordinates: 4343361..4343651 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A17_RS21310 (4338963)	4338963..4339562	+	600	WP_001295269.1	glucose-1-phosphatase	-
A7A17_RS21315 (4339556)	4339556..4340428	+	873	WP_000920762.1	virulence factor BrkB family protein	-
A7A17_RS21320 (4340425)	4340425..4340862	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
A7A17_RS21325 (4340907)	4340907..4341848	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
A7A17_RS21330 (4341912)	4341912..4342820	-	909	WP_001162704.1	alpha/beta hydrolase	-
A7A17_RS21335 (4343049)	4343049..4343360	+	312	WP_000897305.1	hypothetical protein	Toxin
A7A17_RS21340 (4343361)	4343361..4343651	+	291	WP_000356397.1	NadS family protein	Antitoxin
A7A17_RS21345 (4344256)	4344256..4344474	+	219	WP_001295676.1	CopG family transcriptional regulator	-
A7A17_RS21350 (4344693)	4344693..4344935	+	243	WP_001087409.1	protein YiiF	-
A7A17_RS21355 (4345265)	4345265..4346194	-	930	WP_000027706.1	formate dehydrogenase accessory protein FdhE	-
A7A17_RS21360 (4346191)	4346191..4346826	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
A7A17_RS21365 (4346823)	4346823..4347725	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T205111 WP_000897305.1 NZ_CP076264:4343049-4343360 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T205111 NZ_CP101379:c4801368-4801057 [Salmonella enterica]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT205111 WP_000356397.1 NZ_CP076264:4343361-4343651 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT205111 NZ_CP101379:c4801056-4800766 [Salmonella enterica]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAACCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7