Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3542715..3543514 Replicon chromosome
Accession NZ_CP076264
Organism Escherichia coli strain NADC1036

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag A7A17_RS17455 Protein ID WP_000347266.1
Coordinates 3543050..3543514 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag A7A17_RS17450 Protein ID WP_001307405.1
Coordinates 3542715..3543050 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A17_RS17435 (3538500) 3538500..3539270 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
A7A17_RS17440 (3539286) 3539286..3540620 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
A7A17_RS17445 (3540995) 3540995..3542566 + 1572 WP_001273741.1 galactarate dehydratase -
A7A17_RS17450 (3542715) 3542715..3543050 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
A7A17_RS17455 (3543050) 3543050..3543514 + 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
A7A17_RS17460 (3543569) 3543569..3544378 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
A7A17_RS17465 (3544627) 3544627..3545907 + 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
A7A17_RS17470 (3545930) 3545930..3546403 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
A7A17_RS17475 (3546414) 3546414..3547193 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
A7A17_RS17480 (3547183) 3547183..3548061 + 879 WP_001315856.1 PTS N-acetylgalactosamine transporter subunit IID -
A7A17_RS17485 (3548079) 3548079..3548513 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3531840..3543514 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T205108 WP_000347266.1 NZ_CP076264:3543050-3543514 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T205108 NZ_CP101379:c4135972-4135685 [Salmonella enterica]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGTTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCCCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT205108 WP_001307405.1 NZ_CP076264:3542715-3543050 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT205108 NZ_CP101379:c4135698-4135396 [Salmonella enterica]
ATGAGCATGGTTAAACATAAACGCGGCAACGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References