Detailed information of TA system TA205083

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 52705..53128
• Replicon: plasmid p04IncF
• Accession: NZ_CP076263
• Organism: Escherichia coli strain 0-4

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: A7A18_RS24695
• Protein ID: WP_229484968.1
• Coordinates: 52705..52824 (-)
• Length: 40 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	52912..53128 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A18_RS24660 (47770)	47770..47997	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
A7A18_RS24665 (48091)	48091..48777	-	687	WP_000332492.1	PAS domain-containing protein	-
A7A18_RS24670 (48967)	48967..49350	-	384	WP_000124980.1	conjugal transfer relaxosome DNA-binding protein TraM	-
A7A18_RS24675 (49684)	49684..50286	+	603	WP_000243717.1	transglycosylase SLT domain-containing protein	-
A7A18_RS24680 (50581)	50581..51402	-	822	WP_001234477.1	DUF932 domain-containing protein	-
A7A18_RS24685 (51519)	51519..51806	-	288	WP_000107547.1	hypothetical protein	-
A7A18_RS24690 (52106)	52106..52412	+	307	Protein_57	hypothetical protein	-
A7A18_RS24695 (52705)	52705..52824	-	120	WP_229484968.1	Hok/Gef family protein	Toxin
A7A18_RS24700 (52772)	52772..52924	-	153	Protein_59	FlmC family protein	-
- (52912)	52912..53128	-	217	NuclAT_0	-	Antitoxin
- (52912)	52912..53128	-	217	NuclAT_0	-	Antitoxin
- (52912)	52912..53128	-	217	NuclAT_0	-	Antitoxin
- (52912)	52912..53128	-	217	NuclAT_0	-	Antitoxin
A7A18_RS24705 (53097)	53097..53859	-	763	Protein_60	plasmid SOS inhibition protein A	-
A7A18_RS24710 (53856)	53856..54263	-	408	WP_001261250.1	conjugation system SOS inhibitor PsiB	-
A7A18_RS24715 (54424)	54424..54603	+	180	WP_000618107.1	hypothetical protein	-
A7A18_RS24720 (54687)	54687..55079	-	393	WP_000272016.1	plasmid partitioning/stability family protein	-
A7A18_RS24725 (55083)	55083..56057	-	975	WP_021534625.1	plasmid segregation protein ParM	-
A7A18_RS24730 (56296)	56296..56670	-	375	WP_000752650.1	hypothetical protein	-
A7A18_RS24735 (56670)	56670..57302	-	633	WP_000312330.1	ParA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..77652	77652

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-34)

Sequences

Toxin

Protein

• Length: 40 a.a.
• Molecular weight: 4455.25 Da
• Isoelectric Point: 8.2691

>T205083 WP_229484968.1 NZ_CP076263:c52824-52705 [Escherichia coli]
VCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESSGK

Nucleotide

• Length: 120 bp

>T205083 NZ_CP101376:316725-317093 [Salmonella enterica]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAAGCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin

Nucleotide

• Length: 217 bp

>AT205083 NZ_CP076263:c53128-52912 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTCTTTTCAGGAAAAGTGAGTGTGGTCAGCGCGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTATCCTGTCGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTCATTAACCCACGAGGCCTCTGCATGTCTAGTCCAC

Structures

Antitoxin