Detailed information of TA system TA205080

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 13277..13531
• Replicon: plasmid p04IncF
• Accession: NZ_CP076263
• Organism: Escherichia coli strain 0-4

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: Q0H0B2
• Locus tag: A7A18_RS24470
• Protein ID: WP_001300273.1
• Coordinates: 13277..13426 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	13470..13531 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A18_RS24440 (9422)	9422..9577	+	156	Protein_7	IS110 family transposase	-
A7A18_RS24445 (9973)	9973..10224	-	252	WP_001315600.1	HTH domain-containing protein	-
A7A18_RS24450 (11574)	11574..12431	-	858	WP_000131011.1	incFII family plasmid replication initiator RepA	-
A7A18_RS24455 (12424)	12424..12498	-	75	WP_001365705.1	RepA leader peptide Tap	-
A7A18_RS24460 (12547)	12547..12672	+	126	WP_071590472.1	replication protein RepA	-
A7A18_RS24465 (12745)	12745..12993	-	249	WP_000083838.1	replication regulatory protein RepA	-
A7A18_RS24470 (13277)	13277..13426	-	150	WP_001300273.1	Hok/Gef family protein	Toxin
- (13470)	13470..13531	+	62	NuclAT_1	-	Antitoxin
- (13470)	13470..13531	+	62	NuclAT_1	-	Antitoxin
- (13470)	13470..13531	+	62	NuclAT_1	-	Antitoxin
- (13470)	13470..13531	+	62	NuclAT_1	-	Antitoxin
A7A18_RS24475 (14191)	14191..14652	-	462	WP_000760077.1	thermonuclease family protein	-
A7A18_RS24480 (15395)	15395..15598	-	204	WP_001327131.1	hypothetical protein	-
A7A18_RS24485 (15753)	15753..16310	-	558	WP_000139319.1	fertility inhibition protein FinO	-
A7A18_RS24490 (16413)	16413..17273	-	861	WP_000704512.1	alpha/beta hydrolase	-
A7A18_RS24495 (17332)	17332..18078	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..77652	77652

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5554.72 Da
• Isoelectric Point: 8.7678

>T205080 WP_001300273.1 NZ_CP076263:c13426-13277 [Escherichia coli]
MTKYALIGVLAVCATVLCFLLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T205080 NZ_CP076263:c13426-13277 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGGTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTTACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT205080 NZ_CP076263:13470-13531 [Escherichia coli]
TTAAGGTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: Q0H0B2

Antitoxin