Detailed information of TA system TA205078

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4360044..4360646
• Replicon: chromosome
• Accession: NZ_CP076262
• Organism: Escherichia coli strain 0-4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: A7A18_RS21365
• Protein ID: WP_000897305.1
• Coordinates: 4360044..4360355 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A7A18_RS21370
• Protein ID: WP_000356397.1
• Coordinates: 4360356..4360646 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A18_RS21340 (4355958)	4355958..4356557	+	600	WP_001295269.1	glucose-1-phosphatase	-
A7A18_RS21345 (4356551)	4356551..4357423	+	873	WP_000920762.1	virulence factor BrkB family protein	-
A7A18_RS21350 (4357420)	4357420..4357857	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
A7A18_RS21355 (4357902)	4357902..4358843	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
A7A18_RS21360 (4358907)	4358907..4359815	-	909	WP_001162704.1	alpha/beta hydrolase	-
A7A18_RS21365 (4360044)	4360044..4360355	+	312	WP_000897305.1	hypothetical protein	Toxin
A7A18_RS21370 (4360356)	4360356..4360646	+	291	WP_000356397.1	NadS family protein	Antitoxin
A7A18_RS21375 (4361251)	4361251..4361469	+	219	WP_001295676.1	CopG family transcriptional regulator	-
A7A18_RS21380 (4361688)	4361688..4361930	+	243	WP_001087409.1	protein YiiF	-
A7A18_RS21385 (4362260)	4362260..4363189	-	930	WP_000027706.1	formate dehydrogenase accessory protein FdhE	-
A7A18_RS21390 (4363186)	4363186..4363821	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
A7A18_RS21395 (4363818)	4363818..4364720	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T205078 WP_000897305.1 NZ_CP076262:4360044-4360355 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T205078 NZ_CP101375:4364133-4364408 [Salmonella enterica]
ATGAATGCCTCCGGCGTCAGCGTCCGCCATATCAACAGCGAAACCTGCATGACCGCCTGTTACTCACAAATCCCCAGCCA
GCACCTTAAGGGTGACTGGCAGGAAGAAGCGGGATTTGAGACCGGGCACGGCGTCACCGTGAAGATCTCCGAGGGGTGTT
TGATCCTGATTGCAGAGACGGACGAAGTGAGGGATCTACGGAAGGAGCTTTATCAGGTGAAAAAGTCGATGAAGCATATT
AAGGCGGGGGTAAATAATGTGGTGAACGGGGATTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT205078 WP_000356397.1 NZ_CP076262:4360356-4360646 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT205078 NZ_CP101375:c4364398-4363976 [Salmonella enterica]
TTCACCACATTATTTACCCCCGCCTTAATATGCTTCATCGACTTTTTCACCTGATAAAGCTCCTTCCGTAGATCCCTCAC
TTCGTCCGTCTCTGCAATCAGGATCAAACACCCCTCGGAGATCTTCACGGTGACGCCGTGCCCGGTCTCAAATCCCGCTT
CTTCCTGCCAGTCACCCTTAAGGTGCTGGCTGGGGATTTGTGAGTAACAGGCGGTCATGCAGGTTTCGCTGTTGATATGG
CGGACGCTGACGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTATTTATCCGCCGGATGCTGGCTGATTGTGGA
GCTCAGGGTGAGTGAGTATGGGCGCGACATCCTGGCACCGCGCCTCCCTCTCCCCGGCCAGCCCCGGCCGGTGATGAGCA
GCCGGCTGCCGGGGCCGTATTTT

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7