Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3543408..3544207 Replicon chromosome
Accession NZ_CP076262
Organism Escherichia coli strain 0-4

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag A7A18_RS17360 Protein ID WP_000347267.1
Coordinates 3543743..3544207 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag A7A18_RS17355 Protein ID WP_001307405.1
Coordinates 3543408..3543743 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A18_RS17340 (3539193) 3539193..3539963 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
A7A18_RS17345 (3539979) 3539979..3541313 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
A7A18_RS17350 (3541688) 3541688..3543259 + 1572 WP_001273741.1 galactarate dehydratase -
A7A18_RS17355 (3543408) 3543408..3543743 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
A7A18_RS17360 (3543743) 3543743..3544207 + 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
A7A18_RS17365 (3544262) 3544262..3545071 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
A7A18_RS17370 (3545320) 3545320..3546600 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
A7A18_RS17375 (3546623) 3546623..3547096 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
A7A18_RS17380 (3547107) 3547107..3547886 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
A7A18_RS17385 (3547876) 3547876..3548754 + 879 WP_001380079.1 PTS N-acetylgalactosamine transporter subunit IID -
A7A18_RS17390 (3548772) 3548772..3549206 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T205076 WP_000347267.1 NZ_CP076262:3543743-3544207 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T205076 NZ_CP101375:3105882-3106166 [Salmonella enterica]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGGCTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGACACAGAAGCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT205076 WP_001307405.1 NZ_CP076262:3543408-3543743 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT205076 NZ_CP101375:3105644-3105892 [Salmonella enterica]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGCAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References