Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 287965..288506 Replicon chromosome
Accession NZ_CP076262
Organism Escherichia coli strain 0-4

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag A7A18_RS01275 Protein ID WP_000615976.1
Coordinates 287965..288243 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag A7A18_RS01280 Protein ID WP_000729704.1
Coordinates 288246..288506 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A18_RS01260 (285547) 285547..286125 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
A7A18_RS01265 (286331) 286331..287098 + 768 WP_096974658.1 class II glutamine amidotransferase -
A7A18_RS01270 (287069) 287069..287809 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
A7A18_RS01275 (287965) 287965..288243 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
A7A18_RS01280 (288246) 288246..288506 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
A7A18_RS01285 (288692) 288692..289465 + 774 WP_000543899.1 C40 family peptidase -
A7A18_RS01290 (289522) 289522..289878 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
A7A18_RS01295 (289871) 289871..290149 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
A7A18_RS01300 (290254) 290254..291966 - 1713 Protein_252 flagellar biosynthesis protein FlhA -
A7A18_RS01305 (291938) 291938..292723 + 786 WP_000207574.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T205056 WP_000615976.1 NZ_CP076262:c288243-287965 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T205056 NZ_CP101373:2502611-2502895 [Salmonella enterica]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGGCTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGACACAGAAGCTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT205056 WP_000729704.1 NZ_CP076262:c288506-288246 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT205056 NZ_CP101373:2502373-2502621 [Salmonella enterica]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGCAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References