Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type I Classification (family/domain) hok-sok/-
Location 45402..45666 Replicon plasmid pSA31IncI2
Accession NZ_CP076261
Organism Escherichia coli strain WY517-2

Toxin (Protein)


Gene name pndA Uniprot ID U9Z417
Locus tag A7A20_RS23955 Protein ID WP_001387489.1
Coordinates 45402..45554 (-) Length 51 a.a.

Antitoxin (RNA)


Gene name pndB
Locus tag -
Coordinates 45604..45666 (+)

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A20_RS23920 (40519) 40519..41732 + 1214 WP_139371347.1 IS3 family transposase -
A7A20_RS23925 (41774) 41774..43036 + 1263 Protein_45 IncI1-type conjugal transfer membrane protein TraY -
A7A20_RS23930 (43112) 43112..43726 + 615 WP_000578648.1 plasmid IncI1-type surface exclusion protein ExcA -
A7A20_RS23935 (43824) 43824..44033 + 210 WP_001140543.1 hemolysin expression modulator Hha -
A7A20_RS23940 (44242) 44242..44418 + 177 WP_001054904.1 hypothetical protein -
A7A20_RS23945 (44483) 44483..44779 - 297 WP_001275298.1 DinQ-like type I toxin DqlB -
A7A20_RS23950 (45079) 45079..45330 + 252 WP_001291965.1 hypothetical protein -
A7A20_RS23955 (45402) 45402..45554 - 153 WP_001387489.1 Hok/Gef family protein Toxin
- (45604) 45604..45666 + 63 NuclAT_0 - Antitoxin
- (45604) 45604..45666 + 63 NuclAT_0 - Antitoxin
- (45604) 45604..45666 + 63 NuclAT_0 - Antitoxin
- (45604) 45604..45666 + 63 NuclAT_0 - Antitoxin
- (45907) 45907..45962 + 56 NuclAT_1 - -
- (45907) 45907..45962 + 56 NuclAT_1 - -
- (45907) 45907..45962 + 56 NuclAT_1 - -
- (45907) 45907..45962 + 56 NuclAT_1 - -
A7A20_RS23960 (46156) 46156..47109 + 954 WP_021513958.1 hypothetical protein -
A7A20_RS23965 (47327) 47327..48535 + 1209 WP_001383960.1 IncI1-type conjugal transfer protein TrbA -
A7A20_RS23970 (48554) 48554..49624 + 1071 WP_257115099.1 IncI1-type conjugal transfer protein TrbB -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - basG 1..115220 115220


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(7-46)


Sequences


Toxin        


Download         Length: 51 a.a.        Molecular weight: 5761.10 Da        Isoelectric Point: 8.7948

>T205051 WP_001387489.1 NZ_CP076261:c45554-45402 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Download         Length: 153 bp

>T205051 NZ_CP101373:365061-365336 [Salmonella enterica]
ATGGGGCAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGAGCCTGACTGGATTCTTATTTATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin


Download         Length: 63 bp

>AT205051 NZ_CP076261:45604-45666 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB U9Z417


Antitoxin

Download structure file

References