Detailed information of TA system TA205045

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4270841..4271443
• Replicon: chromosome
• Accession: NZ_CP076259
• Organism: Escherichia coli strain WY517-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: A7A20_RS20705
• Protein ID: WP_000897305.1
• Coordinates: 4270841..4271152 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A7A20_RS20710
• Protein ID: WP_000356397.1
• Coordinates: 4271153..4271443 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A20_RS20675 (4265871)	4265871..4266656	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
A7A20_RS20680 (4266755)	4266755..4267354	+	600	WP_001295269.1	glucose-1-phosphatase	-
A7A20_RS20685 (4267348)	4267348..4268220	+	873	WP_000920762.1	virulence factor BrkB family protein	-
A7A20_RS20690 (4268217)	4268217..4268654	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
A7A20_RS20695 (4268699)	4268699..4269640	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
A7A20_RS20700 (4269704)	4269704..4270612	-	909	WP_001162704.1	alpha/beta hydrolase	-
A7A20_RS20705 (4270841)	4270841..4271152	+	312	WP_000897305.1	hypothetical protein	Toxin
A7A20_RS20710 (4271153)	4271153..4271443	+	291	WP_000356397.1	NadS family protein	Antitoxin
A7A20_RS20715 (4272048)	4272048..4272266	+	219	WP_001315930.1	CopG family transcriptional regulator	-
A7A20_RS20720 (4272486)	4272486..4272728	+	243	WP_001087409.1	protein YiiF	-
A7A20_RS20725 (4273002)	4273002..4273982	+	981	WP_000399635.1	IS110-like element IS621 family transposase	-
A7A20_RS20730 (4274337)	4274337..4275266	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
A7A20_RS20735 (4275263)	4275263..4275898	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4273002..4273982	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T205045 WP_000897305.1 NZ_CP076259:4270841-4271152 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T205045 NZ_CP101372:c4558083-4557799 [Salmonella enterica]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT205045 WP_000356397.1 NZ_CP076259:4271153-4271443 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT205045 NZ_CP101372:c4558315-4558073 [Salmonella enterica]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7