Detailed information of TA system TA205042

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3460432..3461231
• Replicon: chromosome
• Accession: NZ_CP076259
• Organism: Escherichia coli strain WY517-2

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: F4VJD3
• Locus tag: A7A20_RS16770
• Protein ID: WP_000347266.1
• Coordinates: 3460767..3461231 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: A7A20_RS16765
• Protein ID: WP_001307405.1
• Coordinates: 3460432..3460767 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A20_RS16750 (3456217)	3456217..3456987	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
A7A20_RS16755 (3457003)	3457003..3458337	-	1335	WP_053292629.1	galactarate/glucarate/glycerate transporter GarP	-
A7A20_RS16760 (3458712)	3458712..3460283	+	1572	WP_053292628.1	galactarate dehydratase	-
A7A20_RS16765 (3460432)	3460432..3460767	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
A7A20_RS16770 (3460767)	3460767..3461231	+	465	WP_000347266.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
A7A20_RS16775 (3461286)	3461286..3462095	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
A7A20_RS16780 (3462344)	3462344..3463624	+	1281	WP_000681921.1	tagatose-bisphosphate aldolase subunit KbaZ	-
A7A20_RS16785 (3463647)	3463647..3464120	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
A7A20_RS16790 (3464131)	3464131..3464910	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
A7A20_RS16795 (3464900)	3464900..3465778	+	879	WP_001315856.1	PTS N-acetylgalactosamine transporter subunit IID	-
A7A20_RS16800 (3465796)	3465796..3466230	+	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3449444..3461231	11787

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17841.18 Da
• Isoelectric Point: 9.4947

>T205042 WP_000347266.1 NZ_CP076259:3460767-3461231 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T205042 NZ_CP101372:3843949-3844167 [Salmonella enterica]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT205042 WP_001307405.1 NZ_CP076259:3460432-3460767 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT205042 NZ_CP101372:3843547-3843921 [Salmonella enterica]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A836NGD2

Antitoxin

• AlphaFold DB: A0A0E0XVC7