Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) symER/SymE(toxin)
Location 4727453..4727865 Replicon chromosome
Accession NZ_CP076245
Organism Escherichia coli O157:H7 strain Balcarce_24.2

Toxin (Protein)


Gene name symE Uniprot ID Q8FA88
Locus tag JKN64_RS23725 Protein ID WP_000132630.1
Coordinates 4727524..4727865 (+) Length 114 a.a.

Antitoxin (RNA)


Gene name symR
Locus tag -
Coordinates 4727453..4727529 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKN64_RS23715 (4724080) 4724080..4725549 + 1470 WP_001302468.1 type I restriction-modification system subunit M -
JKN64_RS23720 (4725549) 4725549..4727303 + 1755 WP_000110082.1 restriction endonuclease subunit S -
- (4727453) 4727453..4727529 - 77 NuclAT_14 - Antitoxin
- (4727453) 4727453..4727529 - 77 NuclAT_14 - Antitoxin
- (4727453) 4727453..4727529 - 77 NuclAT_14 - Antitoxin
- (4727453) 4727453..4727529 - 77 NuclAT_14 - Antitoxin
- (4727453) 4727453..4727529 - 77 NuclAT_15 - Antitoxin
- (4727453) 4727453..4727529 - 77 NuclAT_15 - Antitoxin
- (4727453) 4727453..4727529 - 77 NuclAT_15 - Antitoxin
- (4727453) 4727453..4727529 - 77 NuclAT_15 - Antitoxin
JKN64_RS23725 (4727524) 4727524..4727865 + 342 WP_000132630.1 endoribonuclease SymE Toxin
JKN64_RS23730 (4727912) 4727912..4729075 - 1164 WP_001304006.1 DUF1524 domain-containing protein -
JKN64_RS23735 (4729123) 4729123..4730004 - 882 WP_001304007.1 DUF262 domain-containing protein -
JKN64_RS23740 (4730147) 4730147..4730299 - 153 WP_001418365.1 hypothetical protein -
JKN64_RS23745 (4730442) 4730442..4731854 + 1413 WP_000199295.1 PLP-dependent aminotransferase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - espX6 4719652..4740879 21227


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SymE-like

(20-71)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12294.13 Da        Isoelectric Point: 8.4982

>T204980 WP_000132630.1 NZ_CP076245:4727524-4727865 [Escherichia coli O157:H7]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Download         Length: 342 bp

>T204980 NZ_CP076245:4727524-4727865 [Escherichia coli O157:H7]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin


Download         Length: 77 bp

>AT204980 NZ_CP076245:c4727529-4727453 [Escherichia coli O157:H7]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y5T0

Antitoxin

Download structure file

References