Detailed information of TA system TA204978

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4373497..4374191
• Replicon: chromosome
• Accession: NZ_CP076245
• Organism: Escherichia coli O157:H7 strain Balcarce_24.2

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: JKN64_RS22105
• Protein ID: WP_001263495.1
• Coordinates: 4373497..4373895 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: JKN64_RS22110
• Protein ID: WP_000554757.1
• Coordinates: 4373898..4374191 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (4369084)	4369084..4369164	-	81	NuclAT_11	-	-
- (4369084)	4369084..4369164	-	81	NuclAT_11	-	-
- (4369084)	4369084..4369164	-	81	NuclAT_11	-	-
- (4369084)	4369084..4369164	-	81	NuclAT_11	-	-
JKN64_RS22080 (4369760)	4369760..4370218	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JKN64_RS22085 (4370479)	4370479..4371936	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
JKN64_RS22090 (4371993)	4371993..4372514	-	522	Protein_4317	peptide chain release factor H	-
JKN64_RS22095 (4372513)	4372513..4372716	-	204	Protein_4318	RtcB family protein	-
JKN64_RS22100 (4373035)	4373035..4373487	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
JKN64_RS22105 (4373497)	4373497..4373895	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JKN64_RS22110 (4373898)	4373898..4374191	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JKN64_RS22115 (4374243)	4374243..4375298	-	1056	WP_001226186.1	DNA polymerase IV	-
JKN64_RS22120 (4375369)	4375369..4376154	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
JKN64_RS22125 (4376126)	4376126..4377838	+	1713	Protein_4324	flagellar biosynthesis protein FlhA	-
JKN64_RS22130 (4377983)	4377983..4378480	-	498	Protein_4325	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T204978 WP_001263495.1 NZ_CP076245:c4373895-4373497 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T204978 NZ_CP101310:303839-304114 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACAGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT204978 WP_000554757.1 NZ_CP076245:c4374191-4373898 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT204978 NZ_CP101310:304111-304470 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAGCTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6