Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3267290..3268085 Replicon chromosome
Accession NZ_CP076245
Organism Escherichia coli O157:H7 strain Balcarce_24.2

Toxin (Protein)


Gene name yeeV Uniprot ID B7UP43
Locus tag JKN64_RS16785 Protein ID WP_000854914.1
Coordinates 3267290..3267664 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0B0VS41
Locus tag JKN64_RS16790 Protein ID WP_001280954.1
Coordinates 3267711..3268085 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKN64_RS16745 (3262294) 3262294..3262785 - 492 WP_001301587.1 DUF1097 domain-containing protein -
JKN64_RS16750 (3262887) 3262887..3263441 - 555 WP_001001917.1 molecular chaperone YcdY -
JKN64_RS16755 (3263465) 3263465..3264202 - 738 WP_000283664.1 zinc-binding phosphatase -
JKN64_RS16760 (3264257) 3264257..3265195 - 939 WP_000351284.1 glyoxylate/hydroxypyruvate reductase GhrA -
JKN64_RS16770 (3265666) 3265666..3266508 - 843 WP_001280481.1 DUF4942 domain-containing protein -
JKN64_RS16775 (3266593) 3266593..3266790 - 198 WP_000839282.1 DUF957 domain-containing protein -
JKN64_RS16780 (3266802) 3266802..3267293 - 492 WP_000976853.1 DUF5983 family protein -
JKN64_RS16785 (3267290) 3267290..3267664 - 375 WP_000854914.1 TA system toxin CbtA family protein Toxin
JKN64_RS16790 (3267711) 3267711..3268085 - 375 WP_001280954.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
JKN64_RS16795 (3268248) 3268248..3268469 - 222 WP_001220314.1 DUF987 domain-containing protein -
JKN64_RS16800 (3268532) 3268532..3269008 - 477 WP_001186738.1 RadC family protein -
JKN64_RS16805 (3269024) 3269024..3269509 - 486 WP_000214398.1 antirestriction protein -
JKN64_RS16810 (3269600) 3269600..3270418 - 819 WP_001234682.1 DUF932 domain-containing protein -
JKN64_RS16815 (3270758) 3270758..3271828 - 1071 WP_000102669.1 patatin-like phospholipase family protein -
JKN64_RS16820 (3271825) 3271825..3272730 - 906 WP_000203541.1 diguanylate cyclase regulator RdcB family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - csgB / csgD / csgE / csgF / csgG 3258676..3269509 10833


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14117.17 Da        Isoelectric Point: 7.7761

>T204974 WP_000854914.1 NZ_CP076245:c3267664-3267290 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T204974 NZ_CP101305:c4857670-4857497 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13718.51 Da        Isoelectric Point: 6.6249

>AT204974 WP_001280954.1 NZ_CP076245:c3268085-3267711 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Download         Length: 375 bp

>AT204974 NZ_CP101305:c4857994-4857698 [Escherichia coli]
ATGGAAGGTAAAAAAAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LXR5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0VS41

References