Detailed information of TA system TA204960

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 708612..709411
• Replicon: chromosome
• Accession: NZ_CP076245
• Organism: Escherichia coli O157:H7 strain Balcarce_24.2

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: JKN64_RS03480
• Protein ID: WP_000347273.1
• Coordinates: 708612..709076 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: JKN64_RS03485
• Protein ID: WP_001302819.1
• Coordinates: 709076..709411 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKN64_RS03450 (703613)	703613..704047	-	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
JKN64_RS03455 (704065)	704065..704943	-	879	Protein_680	PTS N-acetylgalactosamine transporter subunit IID	-
JKN64_RS03460 (704933)	704933..705712	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
JKN64_RS03465 (705723)	705723..706196	-	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
JKN64_RS03470 (706219)	706219..707499	-	1281	WP_000681927.1	tagatose-bisphosphate aldolase subunit KbaZ	-
JKN64_RS03475 (707748)	707748..708557	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
JKN64_RS03480 (708612)	708612..709076	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
JKN64_RS03485 (709076)	709076..709411	-	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
JKN64_RS03490 (709560)	709560..711131	-	1572	WP_001273776.1	galactarate dehydratase	-
JKN64_RS03495 (711506)	711506..712840	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
JKN64_RS03500 (712856)	712856..713626	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T204960 WP_000347273.1 NZ_CP076245:c709076-708612 [Escherichia coli O157:H7]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T204960 NZ_CP101305:1105931-1106266 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT204960 WP_001302819.1 NZ_CP076245:c709411-709076 [Escherichia coli O157:H7]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT204960 NZ_CP101305:1105683-1105931 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: V0SSH7