Detailed information of TA system TA204952

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 4745172..4745584
• Replicon: chromosome
• Accession: NZ_CP076243
• Organism: Escherichia coli O157:H7 strain Balcarce_14.2

Toxin (Protein)

• Gene name: symE
• Uniprot ID: Q8FA88
• Locus tag: JKN69_RS23965
• Protein ID: WP_000132630.1
• Coordinates: 4745243..4745584 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	4745172..4745248 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKN69_RS23955 (4741799)	4741799..4743268	+	1470	WP_001302468.1	type I restriction-modification system subunit M	-
JKN69_RS23960 (4743268)	4743268..4745022	+	1755	WP_000110082.1	restriction endonuclease subunit S	-
- (4745172)	4745172..4745248	-	77	NuclAT_14	-	Antitoxin
- (4745172)	4745172..4745248	-	77	NuclAT_14	-	Antitoxin
- (4745172)	4745172..4745248	-	77	NuclAT_14	-	Antitoxin
- (4745172)	4745172..4745248	-	77	NuclAT_14	-	Antitoxin
- (4745172)	4745172..4745248	-	77	NuclAT_15	-	Antitoxin
- (4745172)	4745172..4745248	-	77	NuclAT_15	-	Antitoxin
- (4745172)	4745172..4745248	-	77	NuclAT_15	-	Antitoxin
- (4745172)	4745172..4745248	-	77	NuclAT_15	-	Antitoxin
JKN69_RS23965 (4745243)	4745243..4745584	+	342	WP_000132630.1	endoribonuclease SymE	Toxin
JKN69_RS23970 (4745631)	4745631..4746794	-	1164	WP_001304006.1	DUF1524 domain-containing protein	-
JKN69_RS23975 (4746842)	4746842..4747723	-	882	WP_001304007.1	DUF262 domain-containing protein	-
JKN69_RS23980 (4747866)	4747866..4748018	-	153	WP_001418365.1	hypothetical protein	-
JKN69_RS23985 (4748161)	4748161..4749573	+	1413	WP_000199295.1	PLP-dependent aminotransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12294.13 Da
• Isoelectric Point: 8.4982

>T204952 WP_000132630.1 NZ_CP076243:4745243-4745584 [Escherichia coli O157:H7]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T204952 NZ_CP101303:72815-73102 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTAGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT204952 NZ_CP076243:c4745248-4745172 [Escherichia coli O157:H7]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0E0Y5T0

Antitoxin