Detailed information of TA system TA204950

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4391317..4392011
• Replicon: chromosome
• Accession: NZ_CP076243
• Organism: Escherichia coli O157:H7 strain Balcarce_14.2

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: JKN69_RS22350
• Protein ID: WP_001263495.1
• Coordinates: 4391317..4391715 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: JKN69_RS22355
• Protein ID: WP_000554757.1
• Coordinates: 4391718..4392011 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (4386905)	4386905..4386985	-	81	NuclAT_11	-	-
- (4386905)	4386905..4386985	-	81	NuclAT_11	-	-
- (4386905)	4386905..4386985	-	81	NuclAT_11	-	-
- (4386905)	4386905..4386985	-	81	NuclAT_11	-	-
JKN69_RS22325 (4387581)	4387581..4388039	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JKN69_RS22330 (4388300)	4388300..4389756	+	1457	Protein_4366	cytosol nonspecific dipeptidase	-
JKN69_RS22335 (4389813)	4389813..4390334	-	522	Protein_4367	peptide chain release factor H	-
JKN69_RS22340 (4390333)	4390333..4390536	-	204	Protein_4368	RtcB family protein	-
JKN69_RS22345 (4390855)	4390855..4391307	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
JKN69_RS22350 (4391317)	4391317..4391715	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JKN69_RS22355 (4391718)	4391718..4392011	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JKN69_RS22360 (4392063)	4392063..4393118	-	1056	WP_001226186.1	DNA polymerase IV	-
JKN69_RS22365 (4393189)	4393189..4393974	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
JKN69_RS22370 (4393946)	4393946..4395658	+	1713	Protein_4374	flagellar biosynthesis protein FlhA	-
JKN69_RS22375 (4395803)	4395803..4396300	-	498	Protein_4375	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4353164..4397249	44085

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T204950 WP_001263495.1 NZ_CP076243:c4391715-4391317 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T204950 NZ_CP101302:c5211665-5211390 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT204950 WP_000554757.1 NZ_CP076243:c4392011-4391718 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT204950 NZ_CP101302:c5211393-5211034 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCTAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6