Detailed information of TA system TA204932

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 701043..701842
• Replicon: chromosome
• Accession: NZ_CP076243
• Organism: Escherichia coli O157:H7 strain Balcarce_14.2

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: JKN69_RS03450
• Protein ID: WP_000347273.1
• Coordinates: 701043..701507 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: JKN69_RS03455
• Protein ID: WP_001302819.1
• Coordinates: 701507..701842 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKN69_RS03420 (696044)	696044..696478	-	435	WP_000948831.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
JKN69_RS03425 (696496)	696496..697374	-	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
JKN69_RS03430 (697364)	697364..698143	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
JKN69_RS03435 (698154)	698154..698627	-	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
JKN69_RS03440 (698650)	698650..699930	-	1281	WP_000681927.1	tagatose-bisphosphate aldolase subunit KbaZ	-
JKN69_RS03445 (700179)	700179..700988	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
JKN69_RS03450 (701043)	701043..701507	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
JKN69_RS03455 (701507)	701507..701842	-	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
JKN69_RS03460 (701991)	701991..703562	-	1572	WP_001273776.1	galactarate dehydratase	-
JKN69_RS03465 (703937)	703937..705271	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
JKN69_RS03470 (705287)	705287..706057	+	771	WP_001058231.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T204932 WP_000347273.1 NZ_CP076243:c701507-701043 [Escherichia coli O157:H7]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T204932 NZ_CP101302:c1136761-1136483 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT204932 WP_001302819.1 NZ_CP076243:c701842-701507 [Escherichia coli O157:H7]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT204932 NZ_CP101302:c1137024-1136764 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: V0SSH7