Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) symER/SymE(toxin)
Location 4751804..4752216 Replicon chromosome
Accession NZ_CP076241
Organism Escherichia coli O157:H7 strain Vac_07.1

Toxin (Protein)


Gene name symE Uniprot ID Q8FA88
Locus tag JKN65_RS23970 Protein ID WP_000132630.1
Coordinates 4751875..4752216 (+) Length 114 a.a.

Antitoxin (RNA)


Gene name symR
Locus tag -
Coordinates 4751804..4751880 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKN65_RS23960 (4748431) 4748431..4749900 + 1470 WP_001302468.1 type I restriction-modification system subunit M -
JKN65_RS23965 (4749900) 4749900..4751654 + 1755 WP_000110082.1 restriction endonuclease subunit S -
- (4751804) 4751804..4751880 - 77 NuclAT_14 - Antitoxin
- (4751804) 4751804..4751880 - 77 NuclAT_14 - Antitoxin
- (4751804) 4751804..4751880 - 77 NuclAT_14 - Antitoxin
- (4751804) 4751804..4751880 - 77 NuclAT_14 - Antitoxin
- (4751804) 4751804..4751880 - 77 NuclAT_15 - Antitoxin
- (4751804) 4751804..4751880 - 77 NuclAT_15 - Antitoxin
- (4751804) 4751804..4751880 - 77 NuclAT_15 - Antitoxin
- (4751804) 4751804..4751880 - 77 NuclAT_15 - Antitoxin
JKN65_RS23970 (4751875) 4751875..4752216 + 342 WP_000132630.1 endoribonuclease SymE Toxin
JKN65_RS23975 (4752263) 4752263..4753426 - 1164 WP_001304006.1 DUF1524 domain-containing protein -
JKN65_RS23980 (4753474) 4753474..4754355 - 882 WP_001304007.1 DUF262 domain-containing protein -
JKN65_RS23985 (4754498) 4754498..4754650 - 153 WP_001418365.1 hypothetical protein -
JKN65_RS23990 (4754793) 4754793..4756205 + 1413 WP_000199295.1 PLP-dependent aminotransferase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - espX6 4744003..4765212 21209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SymE-like

(20-71)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12294.13 Da        Isoelectric Point: 8.4982

>T204923 WP_000132630.1 NZ_CP076241:4751875-4752216 [Escherichia coli O157:H7]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Download         Length: 342 bp

>T204923 NZ_CP101292:c4716035-4715700 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 77 bp

>AT204923 NZ_CP076241:c4751880-4751804 [Escherichia coli O157:H7]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y5T0

Antitoxin

Download structure file

References