Detailed information of TA system TA204921

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4398638..4399332
• Replicon: chromosome
• Accession: NZ_CP076241
• Organism: Escherichia coli O157:H7 strain Vac_07.1

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: JKN65_RS22360
• Protein ID: WP_001263495.1
• Coordinates: 4398638..4399036 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: JKN65_RS22365
• Protein ID: WP_000554757.1
• Coordinates: 4399039..4399332 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (4394225)	4394225..4394305	-	81	NuclAT_11	-	-
- (4394225)	4394225..4394305	-	81	NuclAT_11	-	-
- (4394225)	4394225..4394305	-	81	NuclAT_11	-	-
- (4394225)	4394225..4394305	-	81	NuclAT_11	-	-
JKN65_RS22335 (4394901)	4394901..4395359	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JKN65_RS22340 (4395620)	4395620..4397077	+	1458	WP_001293020.1	cytosol nonspecific dipeptidase	-
JKN65_RS22345 (4397134)	4397134..4397655	-	522	Protein_4373	peptide chain release factor H	-
JKN65_RS22350 (4397654)	4397654..4397857	-	204	Protein_4374	RtcB family protein	-
JKN65_RS22355 (4398176)	4398176..4398628	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
JKN65_RS22360 (4398638)	4398638..4399036	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JKN65_RS22365 (4399039)	4399039..4399332	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JKN65_RS22370 (4399384)	4399384..4400439	-	1056	WP_001226186.1	DNA polymerase IV	-
JKN65_RS22375 (4400510)	4400510..4401295	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
JKN65_RS22380 (4401267)	4401267..4402979	+	1713	Protein_4380	flagellar biosynthesis protein FlhA	-
JKN65_RS22385 (4403124)	4403124..4403621	-	498	Protein_4381	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4362574..4404570	41996

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T204921 WP_001263495.1 NZ_CP076241:c4399036-4398638 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T204921 NZ_CP101292:c4324224-4323826 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT204921 WP_000554757.1 NZ_CP076241:c4399332-4399039 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT204921 NZ_CP101292:c4324451-4324224 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: S1FJN6