Detailed information of TA system TA204903

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 717068..717867
• Replicon: chromosome
• Accession: NZ_CP076241
• Organism: Escherichia coli O157:H7 strain Vac_07.1

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: JKN65_RS03555
• Protein ID: WP_000347273.1
• Coordinates: 717068..717532 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: JKN65_RS03560
• Protein ID: WP_001302819.1
• Coordinates: 717532..717867 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKN65_RS03525 (712069)	712069..712503	-	435	WP_000948831.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
JKN65_RS03530 (712521)	712521..713399	-	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
JKN65_RS03535 (713389)	713389..714168	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
JKN65_RS03540 (714179)	714179..714652	-	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
JKN65_RS03545 (714675)	714675..715955	-	1281	WP_000681927.1	tagatose-bisphosphate aldolase subunit KbaZ	-
JKN65_RS03550 (716204)	716204..717013	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
JKN65_RS03555 (717068)	717068..717532	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
JKN65_RS03560 (717532)	717532..717867	-	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
JKN65_RS03565 (718016)	718016..719587	-	1572	WP_001273776.1	galactarate dehydratase	-
JKN65_RS03570 (719962)	719962..721296	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
JKN65_RS03575 (721312)	721312..722082	+	771	WP_001058231.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T204903 WP_000347273.1 NZ_CP076241:c717532-717068 [Escherichia coli O157:H7]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T204903 NZ_CP101292:671868-672242 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT204903 WP_001302819.1 NZ_CP076241:c717867-717532 [Escherichia coli O157:H7]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT204903 NZ_CP101292:671410-671778 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: V0SSH7