Detailed information of TA system TA204892

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4439723..4440417
• Replicon: chromosome
• Accession: NZ_CP076237
• Organism: Escherichia coli O157:H7 strain 146N4

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: JKN67_RS22545
• Protein ID: WP_001263495.1
• Coordinates: 4439723..4440121 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: JKN67_RS22550
• Protein ID: WP_000554757.1
• Coordinates: 4440124..4440417 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (4435310)	4435310..4435390	-	81	NuclAT_11	-	-
- (4435310)	4435310..4435390	-	81	NuclAT_11	-	-
- (4435310)	4435310..4435390	-	81	NuclAT_11	-	-
- (4435310)	4435310..4435390	-	81	NuclAT_11	-	-
JKN67_RS22520 (4435986)	4435986..4436444	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JKN67_RS22525 (4436705)	4436705..4438162	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
JKN67_RS22530 (4438219)	4438219..4438740	-	522	Protein_4406	peptide chain release factor H	-
JKN67_RS22535 (4438739)	4438739..4438942	-	204	Protein_4407	RtcB family protein	-
JKN67_RS22540 (4439261)	4439261..4439713	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
JKN67_RS22545 (4439723)	4439723..4440121	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JKN67_RS22550 (4440124)	4440124..4440417	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JKN67_RS22555 (4440469)	4440469..4441524	-	1056	WP_001226186.1	DNA polymerase IV	-
JKN67_RS22560 (4441595)	4441595..4442380	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
JKN67_RS22565 (4442352)	4442352..4444064	+	1713	Protein_4413	flagellar biosynthesis protein FlhA	-
JKN67_RS22570 (4444209)	4444209..4444706	-	498	Protein_4414	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T204892 WP_001263495.1 NZ_CP076237:c4440121-4439723 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T204892 NZ_CP101275:c133354-133049 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT204892 WP_000554757.1 NZ_CP076237:c4440417-4440124 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT204892 NZ_CP101275:c133574-133356 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: S1FJN6