Detailed information of TA system TA204884

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3188605..3188830
• Replicon: chromosome
• Accession: NZ_CP076237
• Organism: Escherichia coli O157:H7 strain 146N4

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: JKN67_RS16320
• Protein ID: WP_000813254.1
• Coordinates: 3188605..3188760 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3188772..3188830 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKN67_RS28220	3184063..3184194	-	132	WP_001344632.1	DUF3927 family protein	-
JKN67_RS16280	3184191..3184553	-	363	Protein_3179	tellurite resistance TerB family protein	-
JKN67_RS16285	3184791..3185612	-	822	WP_033803818.1	antitermination protein	-
JKN67_RS16290	3185609..3185983	-	375	WP_001121082.1	RusA family crossover junction endodeoxyribonuclease	-
JKN67_RS16295	3185996..3187045	-	1050	WP_001415191.1	DUF968 domain-containing protein	-
JKN67_RS16300	3187047..3187316	-	270	WP_012817785.1	hypothetical protein	-
JKN67_RS16305	3187370..3187564	-	195	WP_000701093.1	hypothetical protein	-
JKN67_RS16310	3187821..3188192	+	372	WP_001217944.1	type II toxin-antitoxin system RelE/ParE family toxin	-
JKN67_RS16315	3188185..3188502	+	318	WP_000042395.1	DNA-binding transcriptional regulator	-
JKN67_RS16320	3188605..3188760	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3188772..3188830	+	59	-	-	Antitoxin
JKN67_RS16325	3189006..3189110	-	105	WP_001278450.1	hypothetical protein	-
JKN67_RS16330	3189226..3189939	-	714	WP_000555106.1	DUF551 domain-containing protein	-
JKN67_RS16335	3190140..3190352	-	213	WP_000063625.1	hypothetical protein	-
JKN67_RS16340	3190401..3190757	-	357	WP_000403779.1	hypothetical protein	-
JKN67_RS16345	3191119..3191454	-	336	Protein_3192	ead/Ea22-like family protein	-
JKN67_RS16350	3191451..3191627	-	177	WP_000753069.1	hypothetical protein	-
JKN67_RS16355	3191620..3191802	-	183	WP_001224662.1	hypothetical protein	-
JKN67_RS16360	3191836..3192048	-	213	WP_000935422.1	hypothetical protein	-
JKN67_RS16365	3192099..3192455	-	357	WP_000403783.1	hypothetical protein	-
JKN67_RS16370	3192433..3192808	-	376	Protein_3197	ead/Ea22-like family protein	-
JKN67_RS16375	3192805..3193101	-	297	WP_001266133.1	DUF4406 domain-containing protein	-
JKN67_RS16380	3193098..3193490	-	393	WP_001141097.1	DUF977 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleC / nleG7'	3148799..3255899	107100

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T204884 WP_000813254.1 NZ_CP076237:c3188760-3188605 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T204884 NZ_CP101274:c3776345-3775938 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT204884 NZ_CP076237:3188772-3188830 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTGTGTAGAGCATAGATATGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin