Detailed information of TA system TA204881

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2920898..2921123
• Replicon: chromosome
• Accession: NZ_CP076237
• Organism: Escherichia coli O157:H7 strain 146N4

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: JKN67_RS15000
• Protein ID: WP_000813258.1
• Coordinates: 2920968..2921123 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2920898..2920956 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKN67_RS14950	2915963..2916205	+	243	WP_000747948.1	Cro/CI family transcriptional regulator	-
JKN67_RS14955	2916189..2916614	+	426	WP_000693878.1	toxin YdaT family protein	-
JKN67_RS14960	2916683..2917726	+	1044	WP_001262402.1	DnaT-like ssDNA-binding domain-containing protein	-
JKN67_RS14965	2917719..2918180	+	462	WP_000139447.1	replication protein P	-
JKN67_RS14970	2918214..2918930	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
JKN67_RS14975	2918963..2919244	+	282	WP_000603384.1	DNA-binding protein	-
JKN67_RS14980	2919241..2919468	+	228	WP_000699809.1	hypothetical protein	-
JKN67_RS14985	2919461..2919772	+	312	WP_001289673.1	hypothetical protein	-
JKN67_RS14990	2919900..2920118	+	219	WP_000683609.1	DUF4014 family protein	-
JKN67_RS14995	2920120..2920677	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2920898..2920956	-	59	-	-	Antitoxin
JKN67_RS15000	2920968..2921123	+	156	WP_000813258.1	Hok/Gef family protein	Toxin
JKN67_RS15005	2921243..2921587	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
JKN67_RS15010	2921709..2921981	+	273	WP_000191872.1	hypothetical protein	-
JKN67_RS15015	2921983..2923032	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
JKN67_RS15020	2923045..2923350	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
JKN67_RS15025	2923413..2923967	+	555	WP_000640035.1	DUF1133 family protein	-
JKN67_RS15030	2924192..2924389	+	198	WP_000917763.1	hypothetical protein	-
JKN67_RS15035	2924525..2925238	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
JKN67_RS15050	2925689..2926120	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2909874..2959920	50046

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T204881 WP_000813258.1 NZ_CP076237:2920968-2921123 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T204881 NZ_CP101274:c2834261-2834010 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCATAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT204881 NZ_CP076237:c2920956-2920898 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin