Detailed information of TA system TA204866

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 708440..709239
• Replicon: chromosome
• Accession: NZ_CP076237
• Organism: Escherichia coli O157:H7 strain 146N4

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: JKN67_RS03485
• Protein ID: WP_000347273.1
• Coordinates: 708440..708904 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: JKN67_RS03490
• Protein ID: WP_001302819.1
• Coordinates: 708904..709239 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKN67_RS03455 (703441)	703441..703875	-	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
JKN67_RS03460 (703893)	703893..704771	-	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
JKN67_RS03465 (704761)	704761..705540	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
JKN67_RS03470 (705551)	705551..706024	-	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
JKN67_RS03475 (706047)	706047..707327	-	1281	WP_000681927.1	tagatose-bisphosphate aldolase subunit KbaZ	-
JKN67_RS03480 (707576)	707576..708385	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
JKN67_RS03485 (708440)	708440..708904	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
JKN67_RS03490 (708904)	708904..709239	-	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
JKN67_RS03495 (709388)	709388..710959	-	1572	WP_001273776.1	galactarate dehydratase	-
JKN67_RS03500 (711334)	711334..712668	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
JKN67_RS03505 (712684)	712684..713454	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T204866 WP_000347273.1 NZ_CP076237:c708904-708440 [Escherichia coli O157:H7]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T204866 NZ_CP101270:63784-64182 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGTGTCAGGGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGCATCAGTTCGGTTACTCTGATGGAGGTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGACGTTCTGGATTACGACGCTGCTGCTGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCTTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAATTGAAGACTGGAGTTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT204866 WP_001302819.1 NZ_CP076237:c709239-708904 [Escherichia coli O157:H7]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT204866 NZ_CP101270:63557-63784 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCTGCCGGAGAGACATGGGACGAATGGTTCGACGGAA
ACAGTGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCTGGTATGCAGGAAAGGGAGTCATTCTGA

Structures

Toxin

• AlphaFold DB: V0SSH7