Detailed information of TA system TA020486

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 2909165..2909778
• Replicon: chromosome
• Accession: NC_002927
• Organism: Bordetella bronchiseptica RB50

Toxin (Protein)

• Gene name: higB
• Uniprot ID: K0MEM3
• Locus tag: AYT36_RS13960
• Protein ID: WP_003811545.1
• Coordinates: 2909467..2909778 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AYT36_RS13955
• Protein ID: WP_003811547.1
• Coordinates: 2909165..2909464 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYT36_RS13945	2905270..2905944	-	675	WP_003811550.1	response regulator transcription factor	-
AYT36_RS13950	2906074..2909151	+	3078	WP_010926623.1	autotransporter SphB2	-
AYT36_RS13955	2909165..2909464	-	300	WP_003811547.1	helix-turn-helix domain-containing protein	Antitoxin
AYT36_RS13960	2909467..2909778	-	312	WP_003811545.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AYT36_RS13965	2909892..2910515	-	624	WP_003811542.1	glutathione S-transferase family protein	-
AYT36_RS13970	2910634..2911587	-	954	WP_010926624.1	LysR family transcriptional regulator	-
AYT36_RS13975	2911703..2912665	+	963	WP_003811538.1	tripartite tricarboxylate transporter substrate binding protein	-
AYT36_RS13980	2912680..2914074	+	1395	WP_010926625.1	arylsulfatase	-
AYT36_RS13985	2914071..2914658	+	588	WP_010926626.1	LamB/YcsF family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (42-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-96)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11810.72 Da
• Isoelectric Point: 8.8245

>T20486 WP_003811545.1 NC_002927:c2909778-2909467 [Bordetella bronchiseptica RB50]
MEFIETPIFTQLITALLSDEEYSRLQQLLIDNPERGDLLRGGGGIRKMRYGRQGTGKRGGIRVIYYWISQDCQIYMLAAY
AKSKKINLTPDEIAALRELVKEL

Nucleotide

• Length: 312 bp

>T20486 NC_002927:c2909778-2909467 [Bordetella bronchiseptica RB50]
ATGGAGTTCATCGAAACCCCAATTTTCACGCAATTGATCACCGCGTTGCTGTCGGATGAGGAATATTCCAGGCTGCAACA
GCTGCTGATCGACAACCCCGAAAGGGGCGATCTGCTGCGTGGGGGCGGCGGTATACGAAAGATGCGTTACGGCCGGCAAG
GCACGGGCAAGCGAGGTGGCATCCGGGTCATTTATTACTGGATCAGCCAGGATTGTCAGATCTACATGCTTGCGGCTTAT
GCAAAGTCGAAGAAGATCAACCTGACCCCGGACGAAATCGCGGCGCTGCGCGAACTGGTCAAGGAGCTGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10946.70 Da
• Isoelectric Point: 11.2467

>AT20486 WP_003811547.1 NC_002927:c2909464-2909165 [Bordetella bronchiseptica RB50]
MKKDNTVFNDLQASLKQAVAIRQGKMPPSRVFKVETVDVKATREKTHMSQTEFAKAIHVSVRTLQNWEQGHRNPTGPAVA
LLKIVASAPDIAVRALRTA

Nucleotide

• Length: 300 bp

>AT20486 NC_002927:c2909464-2909165 [Bordetella bronchiseptica RB50]
ATGAAAAAAGACAACACGGTGTTCAACGACCTGCAAGCCAGCCTGAAGCAAGCCGTGGCCATTCGCCAAGGCAAGATGCC
GCCATCGCGTGTATTCAAGGTTGAGACTGTGGATGTGAAGGCAACGCGCGAAAAAACGCACATGTCTCAAACGGAATTCG
CCAAAGCCATCCACGTCAGTGTCCGTACGCTGCAGAACTGGGAGCAAGGTCACCGCAATCCGACGGGCCCTGCCGTGGCG
CTTCTGAAAATCGTGGCAAGCGCCCCGGACATTGCCGTGCGAGCGCTGCGGACCGCGTAA

Structures

Toxin

• AlphaFold DB: K0MEM3