Detailed information of TA system TA204857

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 4725124..4725536
• Replicon: chromosome
• Accession: NZ_CP076235
• Organism: Escherichia coli O157:H7 strain 9.1_Anguil

Toxin (Protein)

• Gene name: symE
• Uniprot ID: Q8FA88
• Locus tag: JKN68_RS23790
• Protein ID: WP_000132630.1
• Coordinates: 4725195..4725536 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	4725124..4725200 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKN68_RS23780 (4721751)	4721751..4723220	+	1470	WP_001302468.1	type I restriction-modification system subunit M	-
JKN68_RS23785 (4723220)	4723220..4724974	+	1755	WP_000110082.1	restriction endonuclease subunit S	-
- (4725124)	4725124..4725200	-	77	NuclAT_14	-	Antitoxin
- (4725124)	4725124..4725200	-	77	NuclAT_14	-	Antitoxin
- (4725124)	4725124..4725200	-	77	NuclAT_14	-	Antitoxin
- (4725124)	4725124..4725200	-	77	NuclAT_14	-	Antitoxin
- (4725124)	4725124..4725200	-	77	NuclAT_15	-	Antitoxin
- (4725124)	4725124..4725200	-	77	NuclAT_15	-	Antitoxin
- (4725124)	4725124..4725200	-	77	NuclAT_15	-	Antitoxin
- (4725124)	4725124..4725200	-	77	NuclAT_15	-	Antitoxin
JKN68_RS23790 (4725195)	4725195..4725536	+	342	WP_000132630.1	endoribonuclease SymE	Toxin
JKN68_RS23795 (4725583)	4725583..4726746	-	1164	WP_001304006.1	DUF1524 domain-containing protein	-
JKN68_RS23800 (4726794)	4726794..4727675	-	882	WP_001304007.1	DUF262 domain-containing protein	-
JKN68_RS23805 (4727818)	4727818..4727970	-	153	WP_001418365.1	hypothetical protein	-
JKN68_RS23810 (4728113)	4728113..4729525	+	1413	WP_000199295.1	PLP-dependent aminotransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002655 Escherichia coli O157:H7 str. EDL933 5456776..5475497 0.003 View NC_002695 Escherichia coli O157:H7 str. Sakai 5426911..5445631 0.003	inside	Genomic island	-	espX6	4717323..4738556	21233

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12294.13 Da
• Isoelectric Point: 8.4982

>T204857 WP_000132630.1 NZ_CP076235:4725195-4725536 [Escherichia coli O157:H7]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T204857 NZ_CP101269:3564636-3565178 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Nucleotide

• Length: 77 bp

>AT204857 NZ_CP076235:c4725200-4725124 [Escherichia coli O157:H7]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0E0Y5T0

Antitoxin