Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4371224..4371918 Replicon chromosome
Accession NZ_CP076235
Organism Escherichia coli O157:H7 strain 9.1_Anguil

Toxin (Protein)


Gene name yafO Uniprot ID C3TNX7
Locus tag JKN68_RS22175 Protein ID WP_001263495.1
Coordinates 4371224..4371622 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag JKN68_RS22180 Protein ID WP_000554757.1
Coordinates 4371625..4371918 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (4366811) 4366811..4366891 - 81 NuclAT_11 - -
- (4366811) 4366811..4366891 - 81 NuclAT_11 - -
- (4366811) 4366811..4366891 - 81 NuclAT_11 - -
- (4366811) 4366811..4366891 - 81 NuclAT_11 - -
JKN68_RS22150 (4367487) 4367487..4367945 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
JKN68_RS22155 (4368206) 4368206..4369663 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
JKN68_RS22160 (4369720) 4369720..4370241 - 522 Protein_4333 peptide chain release factor H -
JKN68_RS22165 (4370240) 4370240..4370443 - 204 Protein_4334 RtcB family protein -
JKN68_RS22170 (4370762) 4370762..4371214 - 453 WP_001059871.1 GNAT family N-acetyltransferase -
JKN68_RS22175 (4371224) 4371224..4371622 - 399 WP_001263495.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
JKN68_RS22180 (4371625) 4371625..4371918 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
JKN68_RS22185 (4371970) 4371970..4373025 - 1056 WP_001226186.1 DNA polymerase IV -
JKN68_RS22190 (4373096) 4373096..4373881 - 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
JKN68_RS22195 (4373853) 4373853..4375565 + 1713 Protein_4340 flagellar biosynthesis protein FlhA -
JKN68_RS22200 (4375710) 4375710..4376207 - 498 Protein_4341 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15473.86 Da        Isoelectric Point: 8.0949

>T204855 WP_001263495.1 NZ_CP076235:c4371622-4371224 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T204855 NZ_CP101269:2755359-2755466 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT204855 WP_000554757.1 NZ_CP076235:c4371918-4371625 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT204855 NZ_CP101269:c2755309-2755246 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTAAACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References