Detailed information of TA system TA204850

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3484600..3484825
• Replicon: chromosome
• Accession: NZ_CP076235
• Organism: Escherichia coli O157:H7 strain 9.1_Anguil

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: JKN68_RS18015
• Protein ID: WP_000813263.1
• Coordinates: 3484600..3484755 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3484767..3484825 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKN68_RS17980	3480054..3480767	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
JKN68_RS17985	3480905..3481101	-	197	Protein_3515	TrmB family transcriptional regulator	-
JKN68_RS17990	3481388..3482206	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
JKN68_RS17995	3482358..3482729	-	372	WP_000090264.1	antiterminator Q family protein	-
JKN68_RS18000	3482719..3483090	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
JKN68_RS18005	3483103..3484152	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
JKN68_RS18010	3484154..3484432	-	279	WP_001341388.1	hypothetical protein	-
JKN68_RS18015	3484600..3484755	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3484767..3484825	+	59	-	-	Antitoxin
JKN68_RS18020	3485015..3485098	-	84	Protein_3522	ORF6N domain-containing protein	-
JKN68_RS18025	3485360..3486133	+	774	WP_000160654.1	alpha/beta hydrolase	-
JKN68_RS18030	3486485..3486898	-	414	WP_001151233.1	DUF977 family protein	-
JKN68_RS18035	3486914..3487684	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
JKN68_RS18040	3487706..3488452	-	747	WP_000788745.1	ATP-binding protein	-
JKN68_RS18045	3488459..3489550	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T204850 WP_000813263.1 NZ_CP076235:c3484755-3484600 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T204850 NZ_CP101269:2042420-2042523 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Nucleotide

• Length: 59 bp

>AT204850 NZ_CP076235:3484767-3484825 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin