Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 701017..701816 Replicon chromosome
Accession NZ_CP076235
Organism Escherichia coli O157:H7 strain 9.1_Anguil

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag JKN68_RS03450 Protein ID WP_000347273.1
Coordinates 701017..701481 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID C3ST72
Locus tag JKN68_RS03455 Protein ID WP_001302819.1
Coordinates 701481..701816 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKN68_RS03420 (696018) 696018..696452 - 435 WP_000948831.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
JKN68_RS03425 (696470) 696470..697348 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
JKN68_RS03430 (697338) 697338..698117 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
JKN68_RS03435 (698128) 698128..698601 - 474 WP_001301475.1 PTS N-acetylgalactosamine transporter subunit IIB -
JKN68_RS03440 (698624) 698624..699904 - 1281 WP_000681927.1 tagatose-bisphosphate aldolase subunit KbaZ -
JKN68_RS03445 (700153) 700153..700962 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JKN68_RS03450 (701017) 701017..701481 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JKN68_RS03455 (701481) 701481..701816 - 336 WP_001302819.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JKN68_RS03460 (701965) 701965..703536 - 1572 WP_001273776.1 galactarate dehydratase -
JKN68_RS03465 (703911) 703911..705245 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
JKN68_RS03470 (705261) 705261..706031 + 771 WP_001058231.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T204833 WP_000347273.1 NZ_CP076235:c701481-701017 [Escherichia coli O157:H7]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T204833 NZ_CP101263:2734884-2734991 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12388.97 Da        Isoelectric Point: 4.8616

>AT204833 WP_001302819.1 NZ_CP076235:c701816-701481 [Escherichia coli O157:H7]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT204833 NZ_CP101263:c2734837-2734771 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure

References