Detailed information of TA system TA204807

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 3222301..3222672
• Replicon: chromosome
• Accession: NZ_CP076232
• Organism: Escherichia coli O157:H7 strain 7.1_Anguil

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A839BBC2
• Locus tag: XE90_RS15930
• Protein ID: WP_021500490.1
• Coordinates: 3222478..3222672 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	3222301..3222479 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XE90_RS15900 (3218053)	3218053..3218223	+	171	WP_001625136.1	protein YnaL	-
XE90_RS15905 (3218256)	3218256..3219629	+	1374	WP_000123746.1	ATP-dependent RNA helicase DbpA	-
XE90_RS15910 (3219758)	3219758..3220693	-	936	WP_001157377.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
XE90_RS15915 (3220745)	3220745..3221980	-	1236	WP_000040838.1	site-specific integrase	-
XE90_RS15920 (3221982)	3221982..3222197	-	216	WP_000079604.1	excisionase XisR	-
- (3222301)	3222301..3222479	+	179	NuclAT_0	-	Antitoxin
- (3222301)	3222301..3222479	+	179	NuclAT_0	-	Antitoxin
- (3222301)	3222301..3222479	+	179	NuclAT_0	-	Antitoxin
- (3222301)	3222301..3222479	+	179	NuclAT_0	-	Antitoxin
XE90_RS15925 (3222297)	3222297..3222485	-	189	WP_001298826.1	DUF1187 family protein	-
XE90_RS15930 (3222478)	3222478..3222672	-	195	WP_021500490.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
XE90_RS15935 (3222736)	3222736..3223788	-	1053	WP_001004423.1	RecT family recombinase	-
XE90_RS15940 (3223800)	3223800..3226925	-	3126	WP_046426232.1	exodeoxyribonuclease VIII	-
XE90_RS15945 (3227027)	3227027..3227302	-	276	WP_226326710.1	hypothetical protein	-
XE90_RS15950 (3227377)	3227377..3227547	-	171	WP_001359121.1	YdaE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI / nleH2 / nleF / espM1 / paa / nleG7' / espFu/tccP / espJ	3201936..3436491	234555

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7046.98 Da
• Isoelectric Point: 9.2886

>T204807 WP_021500490.1 NZ_CP076232:c3222672-3222478 [Escherichia coli O157:H7]
MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T204807 NZ_CP101257:1020091-1020426 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Nucleotide

• Length: 179 bp

>AT204807 NZ_CP076232:3222301-3222479 [Escherichia coli O157:H7]
GAGGACTGAAGTTGCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTGGTTAATTTTGTTTTTGAGTACCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A839BBC2

Antitoxin