Detailed information of TA system TA204802

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1605553..1606247
• Replicon: chromosome
• Accession: NZ_CP076232
• Organism: Escherichia coli O157:H7 strain 7.1_Anguil

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: XE90_RS07560
• Protein ID: WP_001263495.1
• Coordinates: 1605849..1606247 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: XE90_RS07555
• Protein ID: WP_000554757.1
• Coordinates: 1605553..1605846 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XE90_RS07535 (1601264)	1601264..1601761	+	498	Protein_1466	REP-associated tyrosine transposase RayT	-
XE90_RS07540 (1601906)	1601906..1603618	-	1713	Protein_1467	flagellar biosynthesis protein FlhA	-
XE90_RS07545 (1603590)	1603590..1604375	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
XE90_RS07550 (1604446)	1604446..1605501	+	1056	WP_001226186.1	DNA polymerase IV	-
XE90_RS07555 (1605553)	1605553..1605846	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
XE90_RS07560 (1605849)	1605849..1606247	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
XE90_RS07565 (1606257)	1606257..1606709	+	453	WP_001059871.1	GNAT family N-acetyltransferase	-
XE90_RS07570 (1607028)	1607028..1607231	+	204	Protein_1473	RtcB family protein	-
XE90_RS07575 (1607230)	1607230..1607751	+	522	Protein_1474	peptide chain release factor H	-
XE90_RS07580 (1607808)	1607808..1609265	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
XE90_RS07585 (1609526)	1609526..1609984	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
- (1610580)	1610580..1610660	+	81	NuclAT_12	-	-
- (1610580)	1610580..1610660	+	81	NuclAT_12	-	-
- (1610580)	1610580..1610660	+	81	NuclAT_12	-	-
- (1610580)	1610580..1610660	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T204802 WP_001263495.1 NZ_CP076232:1605849-1606247 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T204802 NZ_CP101257:295137-295367 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT204802 WP_000554757.1 NZ_CP076232:1605553-1605846 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT204802 NZ_CP101257:295364-295723 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6