Detailed information of TA system TA020479

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2537020..2537537
• Replicon: chromosome
• Accession: NC_002758
• Organism: Staphylococcus aureus subsp. aureus Mu50

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A3A3ATA4
• Locus tag: SAV_RS13100
• Protein ID: WP_000113265.1
• Coordinates: 2537020..2537286 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: SAV_RS13105
• Protein ID: WP_000584499.1
• Coordinates: 2537286..2537537 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAV_RS13075	2532448..2532984	-	537	WP_000169317.1	GNAT family N-acetyltransferase	-
SAV_RS13080	2533218..2534042	-	825	WP_000572038.1	formate/nitrite transporter family protein	-
SAV_RS13085	2534259..2534441	-	183	WP_000230292.1	hypothetical protein	-
SAV_RS13090	2534525..2534992	-	468	WP_000153347.1	SRPBCC domain-containing protein	-
SAV_RS13095	2535178..2536725	-	1548	WP_000727772.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
SAV_RS15440	2536914..2536985	-	72	Protein_2462	hypothetical protein	-
SAV_RS13100	2537020..2537286	-	267	WP_000113265.1	Txe/YoeB family addiction module toxin	Toxin
SAV_RS13105	2537286..2537537	-	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
SAV_RS13110	2537702..2537803	-	102	Protein_2465	hypothetical protein	-
SAV_RS13115	2537810..2538409	-	600	WP_000162810.1	DsbA family protein	-
SAV_RS13120	2538428..2538790	-	363	WP_000819843.1	DUF4467 domain-containing protein	-
SAV_RS13125	2539045..2540295	+	1251	WP_001012225.1	FemA/FemB family glycyltransferase FmhA	-
SAV_RS13130	2540392..2541123	-	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
SAV_RS13135	2541120..2541839	-	720	WP_000479576.1	amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-81)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10436.96 Da
• Isoelectric Point: 10.0573

>T20479 WP_000113265.1 NC_002758:c2537286-2537020 [Staphylococcus aureus subsp. aureus Mu50]
MARLNITFSPQAFEDYKYFQQNNKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T20479 NC_002758:c2537286-2537020 [Staphylococcus aureus subsp. aureus Mu50]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACAATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT20479 WP_000584499.1 NC_002758:c2537537-2537286 [Staphylococcus aureus subsp. aureus Mu50]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT20479 NC_002758:c2537537-2537286 [Staphylococcus aureus subsp. aureus Mu50]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• PDB: 7BWF

Antitoxin

• AlphaFold DB: T1YB60