Detailed information of TA system TA204788

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4500455..4501149
• Replicon: chromosome
• Accession: NZ_CP076230
• Organism: Escherichia coli O157:H7 strain Rafaela_II

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: XF37_RS22950
• Protein ID: WP_001263495.1
• Coordinates: 4500455..4500853 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: XF37_RS22955
• Protein ID: WP_000554757.1
• Coordinates: 4500856..4501149 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (4496042)	4496042..4496122	-	81	NuclAT_11	-	-
- (4496042)	4496042..4496122	-	81	NuclAT_11	-	-
- (4496042)	4496042..4496122	-	81	NuclAT_11	-	-
- (4496042)	4496042..4496122	-	81	NuclAT_11	-	-
XF37_RS22925 (4496718)	4496718..4497176	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
XF37_RS22930 (4497437)	4497437..4498894	+	1458	WP_001293020.1	cytosol nonspecific dipeptidase	-
XF37_RS22935 (4498951)	4498951..4499472	-	522	Protein_4491	peptide chain release factor H	-
XF37_RS22940 (4499471)	4499471..4499674	-	204	Protein_4492	RtcB family protein	-
XF37_RS22945 (4499993)	4499993..4500445	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
XF37_RS22950 (4500455)	4500455..4500853	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
XF37_RS22955 (4500856)	4500856..4501149	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
XF37_RS22960 (4501201)	4501201..4502256	-	1056	WP_001226186.1	DNA polymerase IV	-
XF37_RS22965 (4502327)	4502327..4503112	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
XF37_RS22970 (4503084)	4503084..4504796	+	1713	Protein_4498	flagellar biosynthesis protein FlhA	-
XF37_RS22975 (4504941)	4504941..4505438	-	498	Protein_4499	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T204788 WP_001263495.1 NZ_CP076230:c4500853-4500455 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T204788 NZ_CP101251:1019977-1020312 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT204788 WP_000554757.1 NZ_CP076230:c4501149-4500856 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT204788 NZ_CP101251:1019729-1019977 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6