Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 3363275..3364070 | Replicon | chromosome |
| Accession | NZ_CP076230 | ||
| Organism | Escherichia coli O157:H7 strain Rafaela_II | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | B7UP43 |
| Locus tag | XF37_RS17495 | Protein ID | WP_000854914.1 |
| Coordinates | 3363275..3363649 (-) | Length | 125 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | A0A0B0VS41 |
| Locus tag | XF37_RS17500 | Protein ID | WP_001280954.1 |
| Coordinates | 3363696..3364070 (-) | Length | 125 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| XF37_RS17455 (3358279) | 3358279..3358770 | - | 492 | WP_001301587.1 | DUF1097 domain-containing protein | - |
| XF37_RS17460 (3358872) | 3358872..3359426 | - | 555 | WP_001001917.1 | molecular chaperone YcdY | - |
| XF37_RS17465 (3359450) | 3359450..3360187 | - | 738 | WP_000283664.1 | zinc-binding phosphatase | - |
| XF37_RS17470 (3360242) | 3360242..3361180 | - | 939 | WP_000351284.1 | glyoxylate/hydroxypyruvate reductase GhrA | - |
| XF37_RS17480 (3361651) | 3361651..3362493 | - | 843 | WP_001280481.1 | DUF4942 domain-containing protein | - |
| XF37_RS17485 (3362578) | 3362578..3362775 | - | 198 | WP_000839282.1 | DUF957 domain-containing protein | - |
| XF37_RS17490 (3362787) | 3362787..3363278 | - | 492 | WP_000976853.1 | DUF5983 family protein | - |
| XF37_RS17495 (3363275) | 3363275..3363649 | - | 375 | WP_000854914.1 | TA system toxin CbtA family protein | Toxin |
| XF37_RS17500 (3363696) | 3363696..3364070 | - | 375 | WP_001280954.1 | type IV toxin-antitoxin system YeeU family antitoxin | Antitoxin |
| XF37_RS17505 (3364233) | 3364233..3364454 | - | 222 | WP_001220314.1 | DUF987 domain-containing protein | - |
| XF37_RS17510 (3364517) | 3364517..3364993 | - | 477 | WP_001186738.1 | RadC family protein | - |
| XF37_RS17515 (3365009) | 3365009..3365494 | - | 486 | WP_000214398.1 | antirestriction protein | - |
| XF37_RS17520 (3365585) | 3365585..3366403 | - | 819 | WP_001234682.1 | DUF932 domain-containing protein | - |
| XF37_RS17525 (3366743) | 3366743..3367813 | - | 1071 | WP_000102669.1 | patatin-like phospholipase family protein | - |
| XF37_RS17530 (3367810) | 3367810..3368715 | - | 906 | WP_000203541.1 | diguanylate cyclase regulator RdcB family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| inside | Genomic island | - | csgB / csgD / csgE / csgF / csgG | 3354661..3365494 | 10833 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 125 a.a. Molecular weight: 14117.17 Da Isoelectric Point: 7.7761
>T204783 WP_000854914.1 NZ_CP076230:c3363649-3363275 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
Download Length: 375 bp
>T204783 NZ_CP101251:295136-295366 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA
Antitoxin
Download Length: 125 a.a. Molecular weight: 13718.51 Da Isoelectric Point: 6.6249
>AT204783 WP_001280954.1 NZ_CP076230:c3364070-3363696 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS
Download Length: 375 bp
>AT204783 NZ_CP101251:295363-295722 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7U9LXR5 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0B0VS41 |