Detailed information of TA system TA020471

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-SprF1/-
• Location: 2087948..2088255
• Replicon: chromosome
• Accession: NC_002758
• Organism: Staphylococcus aureus subsp. aureus Mu50

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: Q2FWU9
• Locus tag: SAV_RS15330
• Protein ID: WP_011447039.1
• Coordinates: 2088079..2088255 (-)
• Length: 59 a.a.

Antitoxin (RNA)

Gene name	SprF1
Locus tag	-
Coordinates	2087948..2088087 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAV_RS10625	2083515..2083694	+	180	WP_000669791.1	hypothetical protein	-
SAV_RS10635	2084005..2084265	+	261	WP_001791826.1	hypothetical protein	-
SAV_RS10640	2084317..2084667	-	351	WP_000702263.1	complement inhibitor SCIN-A	-
SAV_RS10645	2085178..2085513	-	336	Protein_1990	SH3 domain-containing protein	-
SAV_RS10650	2086164..2086655	-	492	WP_000920038.1	staphylokinase	-
SAV_RS10655	2086870..2087601	-	732	WP_000861035.1	CHAP domain-containing protein	-
SAV_RS10660	2087613..2087867	-	255	WP_000611512.1	phage holin	-
SAV_RS10665	2087919..2088026	+	108	WP_031762631.1	hypothetical protein	-
-	2087948..2088087	+	140	NuclAT_0	-	Antitoxin
-	2087948..2088087	+	140	NuclAT_0	-	Antitoxin
-	2087948..2088087	+	140	NuclAT_0	-	Antitoxin
-	2087948..2088087	+	140	NuclAT_0	-	Antitoxin
SAV_RS15330	2088079..2088255	-	177	WP_011447039.1	putative holin-like toxin	Toxin
SAV_RS10675	2088364..2089137	-	774	WP_000750412.1	staphylococcal enterotoxin type A	-
SAV_RS10680	2089510..2089884	-	375	WP_000340977.1	hypothetical protein	-
SAV_RS10685	2089940..2090227	-	288	WP_001262621.1	hypothetical protein	-
SAV_RS10690	2090273..2090425	-	153	WP_001000058.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	scn / sak / sea / hlb / sell / sec / tsst-1 / groEL	2084317..2150747	66430

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (30-58)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6849.45 Da
• Isoelectric Point: 10.6777

>T20471 WP_011447039.1 NC_002758:c2088255-2088079 [Staphylococcus aureus subsp. aureus Mu50]
MDRWWLSEYKEVVPMVALLKSLERRRLMITISTMLQFGLFLIALIGLVIKLIELSNKK

Nucleotide

• Length: 177 bp

>T20471 NC_002758:c2088255-2088079 [Staphylococcus aureus subsp. aureus Mu50]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Nucleotide

• Length: 140 bp

>AT20471 NC_002758:2087948-2088087 [Staphylococcus aureus subsp. aureus Mu50]
ATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAGTGAGCCCGTTAAAAAGACGGTGGCTATTTTAGATTAAA
GATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGATGGTTATTTTTT

Structures

Toxin

• PDB: 7NS1

Antitoxin